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Literature summary extracted from

  • Williams, D.C.; Wildung, M.R.; Jin, A.Q.; Dalal, D.; Oliver, J.S.; Coates, R.M.; Croteau, R.
    Heterologous xpression and characterization of a "Pseudomature" form of taxadiene synthase involved in paclitaxel (taxol) biosynthesis and evaluation of a potential intermediate and inhibitors of the multistep diterpene cyclization reaction (2000), Arch. Biochem. Biophys., 379, 137-146.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.17 medicine chemotherapeutic agent against a range of cancers, including ovarian and breast cancer Taxus brevifolia

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.17 overexpression in Escherichia coli Taxus brevifolia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.17 0.003
-
geranylgeranyl diphosphate native enzyme Taxus brevifolia
4.2.3.17 0.0031
-
geranylgeranyl diphosphate thioredoxin fusion Taxus brevifolia
4.2.3.17 0.0105
-
geranylgeranyl diphosphate M1 preprotein Taxus brevifolia
4.2.3.17 0.014
-
geranylgeranyl diphosphate M93 Taxus brevifolia
4.2.3.17 0.0159
-
geranylgeranyl diphosphate M79 Taxus brevifolia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.17 geranylgeranyl diphosphate Taxus brevifolia catalyzes the first step in taxol biosynthesis taxa-4,11-diene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.17 Taxus brevifolia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.17
-
Taxus brevifolia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.17 cyclopropylidene
-
Taxus brevifolia ?
-
?
4.2.3.17 geranylgeranyl diphosphate
-
Taxus brevifolia taxa-4(5),11(12)-diene + diphosphate
-
?
4.2.3.17 geranylgeranyl diphosphate taxa-4(20),11(12)-diene is formed as a small amount Taxus brevifolia taxa-4(20),11(12)-diene + diphosphate
-
?
4.2.3.17 geranylgeranyl diphosphate catalyzes the first step in taxol biosynthesis Taxus brevifolia taxa-4,11-diene + diphosphate
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.17 0.00004
-
geranylgeranyl diphosphate M1 preprotein Taxus brevifolia
4.2.3.17 0.0000417
-
geranylgeranyl diphosphate thioredoxin fusion Taxus brevifolia
4.2.3.17 0.000133
-
geranylgeranyl diphosphate M93 Taxus brevifolia
4.2.3.17 0.00017
-
geranylgeranyl diphosphate M79 Taxus brevifolia
4.2.3.17 0.000177
-
geranylgeranyl diphosphate native enzyme Taxus brevifolia