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Literature summary extracted from

  • Ritte, G.; Lloyd, J.R.; Eckermann, N.; Rottmann, A.; Kossmann, J.; Steup, M.
    The starch-related R1 protein is an alpha-glucan, water dikinase (2002), Proc. Natl. Acad. Sci. USA, 99, 7166-7171.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.4 expressed in Escherichia coli Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.4 0.00023
-
 ATP
-
 Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.4 Mg2+ essential for activity Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.4 ATP + alpha-glucan + H2O Solanum tuberosum
-
 AMP + phospho-alpha-glucan + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.4 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.4 recombinant enzyme, homogenous Solanum tuberosum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.9.4 ATP + alpha-glucan + H2O = AMP + phospho-alpha-glucan + phosphate ping-pong reaction mechanism Solanum tuberosum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.4 ATP + alpha-glucan + H2O
-
 Solanum tuberosum AMP + phospho-alpha-glucan + phosphate
-
 ?
2.7.9.4 ATP + alpha-glucan + H2O enzyme catalyzes the phophorylation of both C-6 and C-3 position of the glucose residues Solanum tuberosum AMP + phospho-alpha-glucan + phosphate
-
 ?
2.7.9.4 ATP + amylopectin + H2O
-
 Solanum tuberosum AMP + phospho-amylopectin + phosphate
-
 ?
2.7.9.4 ATP + elongated glucogen + H2O
-
 Solanum tuberosum AMP + elongated phosphoglucogen + phosphate
-
 ?
2.7.9.4 ATP + postelongated glycogen + H2O
-
 Solanum tuberosum AMP + postelongated phospho-glycogen + phosphate
-
 ?
2.7.9.4 additional information autocatalytic phosphorylation with beta not with gamma-phosphate Solanum tuberosum ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.4 7
-
-
 Solanum tuberosum