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Literature summary extracted from

  • Olivera, A.; Kohama, T.; Tu, Z.; Milstien, S.; Spiegel, S.
    Purification and characterization of rat kidney sphingosine kinase (1998), J. Biol. Chem., 273, 12576-12583.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.91 D,L-threo-dihydrosphingosine
-
 Rattus norvegicus
2.7.1.91 N,N-dimethylsphingosine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.91 0.005
-
 D-(+)-erythro-sphinganine
-
 Rattus norvegicus
2.7.1.91 0.093
-
 ATP
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.91 Mg2+ maximal activity at 5-10 mM Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.91 49000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.91 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + D-(+)-erythro-sphinganine
-
 Rattus norvegicus ADP + D-(+)-erythro-sphinganine 1-phosphate
-
 ?
2.7.1.91 ATP + sphinganine
-
 Rattus norvegicus ADP + sphinganine 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.91 monomer 1 * 49000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 6.6 7.5
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.91 0.005
-
 D,L-threo-dihydrosphingosine
-
 Rattus norvegicus
2.7.1.91 0.01
-
 N,N-dimethylsphingosine
-
 Rattus norvegicus