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Literature summary extracted from

  • Pitson, S.M.; D'Andrea R.J.; Vandeleur, L.; Moretti, P.A.; Xia, P.; Gamble, J.R.; Vadas, M.A.; Wattenberg, B.W.
    Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes (2000), Biochem. J., 350, 429-441.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91
-
 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.91 ADP
-
 Homo sapiens
2.7.1.91 dihydrosphingosine
-
 Homo sapiens
2.7.1.91 DL-threo-dihydrosphingosine
-
 Homo sapiens
2.7.1.91 EDTA
-
 Homo sapiens
2.7.1.91 N,N,N-trimethylsphingosine
-
 Homo sapiens
2.7.1.91 N,N-dimethylsphingosine
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.91 0.016
-
 D-erythrosphinganine as bovine serum albumin complex Homo sapiens
2.7.1.91 0.02
-
 DL-erythro-dihydrosphingosine
-
 Homo sapiens
2.7.1.91 0.077
-
 ATP
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.91 Ca2+ less stimulating than Mg2+, Mn2+ Homo sapiens
2.7.1.91 Mg2+ highest activity with Homo sapiens
2.7.1.91 Mn2+ less stimulating than Mg2+ Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Homo sapiens Q9NYA1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.91
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + DL-erythro-dihydrosphingosine
-
 Homo sapiens ADP + DL-erythro-dihydrosphingosine 1-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.91 37
-
 10% glycerol, 0.05% Triton X-100, 0.5 M NaCl, stable for 30 min Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.91 7.4
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.91 6.8 7.4 greater than 60% of maximum activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.91 0.006
-
 D,L-threo-dihydrosphingosine
-
 Homo sapiens
2.7.1.91 0.008
-
 N,N-dimethylsphingosine
-
 Homo sapiens
2.7.1.91 1
-
 ADP
-
 Homo sapiens