Literature summary extracted from

Kee, S.M.; Graves, D.J.
Isolation and properties of the active gamma subunit of phosphorylase kinase (1986), J. Biol. Chem., 261, 4732-4737.

General Stability

EC Number	General Stability	Organism
2.7.11.19	Inactive gamma subunit after reverse-phase HPLC can be reactivated by dilution into ice-cold, pH 8.2, Ca2+/calmodulin containing buffer	Oryctolagus cuniculus

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.7.11.19	Ca2+	-	Oryctolagus cuniculus
2.7.11.19	Mg2+	requirement	Oryctolagus cuniculus
2.7.11.19	Mg2+	major role of Mg2+: cosubstrate in Mg2+-ATP complex	Oryctolagus cuniculus
2.7.11.19	phosphate	requirement, phosphate containing enzyme	Oryctolagus cuniculus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.11.19	Oryctolagus cuniculus	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.7.11.19	active gamma subunit from inactive form by reverse-phase HPLC)	Oryctolagus cuniculus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.7.11.19	skeletal muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.11.19	ATP + phosphorylase b	cosubstrate: Mg-ATP complex	Oryctolagus cuniculus	ADP + phosphorylase a	-	?

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.7.11.19	30	-	assay at	Oryctolagus cuniculus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)