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Literature summary extracted from

  • Lanciotti, R.A.; Bender, P.K.
    Baculovirus-directed expression of the gamma-subunit of phosphorylase kinase: purification and calmodulin dependence (1994), Biochem. J., 299, 183-189.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.19 mouse catalytic gamma subunit, Baculovirus-directed expression in Sf9 insect cells Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 0.014
-
 phosphorylase b recombinant gamma subunit, pH 6.8 Mus musculus
2.7.11.19 0.03 0.037 ATP phosphorylase b Mus musculus
2.7.11.19 0.03 0.037 ATP recombinant gamma subunit, pH 8.2 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+ requirement Mus musculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Mus musculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 recombinant gamma-subunit, as expressed in Sf-9 cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Mus musculus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Mus musculus ADP + phosphorylase a
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 Calmodulin additional activation Mus musculus
2.7.11.19 Calmodulin activation of recombinant gamma subunit at pH 6.8, slightly at pH 8.2 Mus musculus