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Literature summary extracted from

  • Killilea, S.D.; Ky, N.M.
    Purification and partial characterization of bovine heart phosphorylase kinase (1983), Arch. Biochem. Biophys., 221, 333-342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 Catalytic subunit of cAMP-dependent protein kinase activation of nonactivated enzyme Bos taurus
2.7.11.19 glycogen activation Bos taurus
2.7.11.19 glycogen effect on nonactivated and activated enzyme Bos taurus
2.7.11.19 additional information the nonactivated enzyme is activated either by limited proteolysis Bos taurus
2.7.11.19 Trypsin proteolytic activation of nonactivated enzyme Bos taurus
2.7.11.19 Trypsin increase of pH 6.8 activity, not pH 8.2 activity Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 additional information
-
 additional information influence on kinetic parameters, glycogen decreases Km-values for phosphorylase b Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+ requirement Bos taurus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Bos taurus
2.7.11.19 phosphate requirement, phosphate containing enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 additional information
-
 MW of trypsinized enzyme Bos taurus
2.7.11.19 18000
-
 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE Bos taurus
2.7.11.19 46000
-
 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE Bos taurus
2.7.11.19 130000
-
 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE Bos taurus
2.7.11.19 140000
-
 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 nonactivated enzyme Bos taurus
2.7.11.19 to near homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 cardiac muscle
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 8.3
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.19 Frozen in liquid N2, in 50 mM beta-glycerophosphate, pH 7, 2 mM EDTA, 1 mM DTT, 10% sucrose, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Bos taurus ADP + phosphorylase a
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 hexadecamer 4 * 140000 + 4 * 130000 + 4 * 46000 + 4 * 18000, (alphabetagammadelta)4, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 additional information
-
 nonactivated enzyme has only low activity at pH 6.8 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 Calmodulin no additional activation Bos taurus