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Literature summary extracted from

  • Chan, K.F.J.; Graves, D.J.
    Rabbit skeletal muscle phosphorylase kinase. Interactions between subunits and influence of calmodulin on different complexes (1982), J. Biol. Chem., 257, 5956-5961.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
2.7.11.19 Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.11.19 Delta-Subunit remains tightly bound to alphagammadelta subunit complex even in the presence of 8 M urea Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 EGTA together with trifluoperazime additive effect Oryctolagus cuniculus
2.7.11.19 Trifluoperazine nonspecific inactivation, at high concentrations, together with EGTA additive effect Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 additional information
-
 additional information effect of isolated delta subunit on kinetic parameters of nonactivated holoenzyme and alphagammadelta complex Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 by affinity chromatography on calmodulin-Sepharose 4B Oryctolagus cuniculus
2.7.11.19 catalytically active alphagammadelta complexes Oryctolagus cuniculus
2.7.11.19 catalytically active gammadelta complexes Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + synthetic pentadecapeptide from amino-terminal of glycogen synthase, i.e. Pro-Leu-Ser-Arg-Thr-Leu-Ser-Val-Ser-Ser-Leu-Pro-Gly-Leu-Glu Oryctolagus cuniculus ?
-
 ?
2.7.11.19 ATP + synthetic tetradecapeptide i.e. Ser-Asp-Gln-Glu-Lys-Arg-Lys-Gln-Ile-Ser-Val-Arg-Gly-Leu Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 More molecular interaction and subunit structure Oryctolagus cuniculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.11.19 37
-
 50% loss of nonactivated enzyme activity within 15 min, 50% loss of alphagammadelta subunit complex activity within 7 min, 90% loss of gammadelta subunit complex activity within 5 min Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 Calmodulin requirement Oryctolagus cuniculus
2.7.11.19 Calmodulin calmodulin containing enzyme i.e. tightly bound delta subunit Oryctolagus cuniculus
2.7.11.19 Calmodulin activation of nonactivated enzyme and alphagammadelta subunit complex Oryctolagus cuniculus
2.7.11.19 Calmodulin interacts with alpha subunit Oryctolagus cuniculus