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Literature summary extracted from

  • Graves, D.J.; Hayakawa, T.; Horvitz, R.A.; Beckman, E.; Krebs, E.G.
    Studies on the subunit structure of trypsin-activated phosphorylase kinase (1973), Biochemistry, 12, 580-585.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
2.7.11.19 Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.11.19 Effects of protein concentration, buffer and ATP on stability and dissociation behaviour of trypsin-activated enzyme Oryctolagus cuniculus
2.7.11.19 In the presence of ATP nonactivated enzyme does not dissociate into catalytically active subunits as trypsin-activated enzyme does Oryctolagus cuniculus
2.7.11.19 Nonactivated or protein kinase-activated enzyme stable in the cold, not trypsin-activated enzyme Oryctolagus cuniculus
2.7.11.19 unstable in dilute solutions Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Storage Stability

EC Number Storage Stability Organism
2.7.11.19 0°C, 2 mg enzyme/ml, trypsin-activated enzyme, with or without ATP, 30% loss of activity within 4 h Oryctolagus cuniculus
2.7.11.19 20°C, 2 mg enzyme/ml, trypsin-activated enzyme, with or without ATP, 4 h Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.11.19 20
-
 2 mg enzyme/ml, trypsin-activated enzyme, with or without ATP, 4 h stable Oryctolagus cuniculus