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Literature summary extracted from
- Purification and characterisation of an unusually heat-stable and acid/base-stable class I fructose-1,6-bisphosphate aldolase from Staphylococcus aureus (1980), Eur. J. Biochem., 108, 295-301.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.13 | EDTA | insensitive | Staphylococcus aureus |  |
| 4.1.2.13 | glycerone phosphate | inhibition in presence of glycerone phosphate and NaBH4 | Staphylococcus aureus | |
| 4.1.2.13 | NaBH4 | inhibition in presence of glycerone phosphate and NaBH4 | Staphylococcus aureus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.13 | 0.045 | - |
D-fructose 1,6-bisphosphate | - |
Staphylococcus aureus | |
| 4.1.2.13 | 25 | - |
fructose 1-phosphate | - |
Staphylococcus aureus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.13 | Co2+ | no effect | Staphylococcus aureus | |
| 4.1.2.13 | Fe2+ | no effect | Staphylococcus aureus | |
| 4.1.2.13 | Mn2+ | no effect | Staphylococcus aureus | |
| 4.1.2.13 | Zn2+ | no effect | Staphylococcus aureus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 33000 | - |
1 * 33000, SDS-PAGE | Staphylococcus aureus |
| 4.1.2.13 | 34000 | - |
sucrose density gradient centrifugation | Staphylococcus aureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.13 | Staphylococcus aureus | - |
ATCC 12600 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.13 | - |
Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | additional information | - |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.13 | D-fructose 1,6-bisphosphate | - |
Staphylococcus aureus | glycerone phosphate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.2.13 | D-Fructose 1-phosphate | - |
Staphylococcus aureus | Glycerone phosphate + D-glyceraldehyde | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.13 | monomer | 1 * 33000, SDS-PAGE | Staphylococcus aureus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 37 | - |
- |
Staphylococcus aureus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 32 | 43 | 32°C: about 30% of maximal activity, 43°C: about 10% of maximal activity | Staphylococcus aureus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 97 | - |
90 min, stable | Staphylococcus aureus |
| 4.1.2.13 | 120 | - |
20 min, about 20% loss of activity | Staphylococcus aureus |
| 4.1.2.13 | 130 | - |
10 min, complete inactivation | Staphylococcus aureus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 6.5 | - |
- |
Staphylococcus aureus |
| 4.1.2.13 | 7.5 | 9 | - |
Staphylococcus aureus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 5.5 | 9 | pH 5.5: about 25% of maximal activity, pH 9.0: about 75% of maximal activity | Staphylococcus aureus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.13 | 1 | 12 | 5 min, stable | Staphylococcus aureus |
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