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Literature summary extracted from

  • Fernandez-Sousa, J.M.; Gavilanes, F.G.; Gavilanes, J.G.; Paredes, J.A.
    D-Fructose 1,6-bisphosphate aldolase from the dipteran Ceratitis capitata (1978), Arch. Biochem. Biophys., 188, 456-465.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0043
-
 D-fructose 1,6-bisphosphate
-
 Ceratitis capitata
4.1.2.13 0.057
-
 D-glyceraldehyde 3-phosphate
-
 Ceratitis capitata
4.1.2.13 0.065
-
 glycerone phosphate
-
 Ceratitis capitata
4.1.2.13 1.5
-
 fructose 1-phosphate
-
 Ceratitis capitata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 30000
-
 4 * 30000, SDS-PAGE Ceratitis capitata
4.1.2.13 159000
-
 gel filtration Ceratitis capitata

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Ceratitis capitata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13
-
 Ceratitis capitata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.13 10.9
-
-
 Ceratitis capitata
4.1.2.13 11
-
-
 Ceratitis capitata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Ceratitis capitata glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-Fructose 1-phosphate
-
 Ceratitis capitata Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.13 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 Ceratitis capitata D-Fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.13 tetramer 4 * 30000, SDS-PAGE Ceratitis capitata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.13 additional information
-
 phosphate and 2-mercaptoethanol protect against thermal inactivation Ceratitis capitata
4.1.2.13 37
-
 pH 7.4, 30 min, stable Ceratitis capitata
4.1.2.13 65
-
 10 min, complete inactivation Ceratitis capitata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 7
-
 cleavage of fructose 1-phosphate Ceratitis capitata
4.1.2.13 7.4
-
 cleavage of D-fructose 1,6-bisphosphate Ceratitis capitata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.13 5.5 9 pH 5.5: about 40% of maximal activity, pH 9.0: about 30% of maximal activity Ceratitis capitata
4.1.2.13 6 9.5 about 30% of maximal activity at pH 6.0 and at pH 9.5, D-fructose 1,6-bisphosphate cleavage Ceratitis capitata