EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | rhodopsin | activates | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | additional information | not inhibited by a synthetic peptide corresponding to the major or minor autophosphorylation site of RK | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.11.14 | membrane | rod membranes | Bos taurus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.14 | Mg2+ | - |
Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 65000 | - |
x * 65000, dephosphorylated RK, SDS-PAGE | Bos taurus |
2.7.11.14 | 66000 | - |
x * 66000, autophosphorylated RK, SDS-PAGE | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + rhodopsin | Bos taurus | second messenger-independent protein kinase, involved in the deactivation of photolyzed rhodopsin | ADP + phosphorhodopsin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.14 | Bos taurus | - |
- |
- |
2.7.11.14 | Bos taurus | P28327 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.14 | additional information | putative posttranslational modification sites: C-terminal isoprenylation, N-terminal myristoylation | Bos taurus |
2.7.11.14 | phosphoprotein | autophosphorylation sites of RK, major sites are Ser-488 and Thr-489, minor site is Ser-21 | Bos taurus |
2.7.11.14 | phosphoprotein | autophosphorylation may lower the affinity of RK for photoactivated rhodopsin via repulsion between phosphorylated sites on photoactivated rhodopsin and RK | Bos taurus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.14 | - |
Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.14 | eye | retina | Bos taurus | - |
2.7.11.14 | retina | rod cell outer segment | Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 0.555 | - |
pH 7.5 | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.14 | ATP + peptide | low catalytic efficiency of RK toward a peptide containing its major autophosphorylation site | Bos taurus | ADP + phosphopeptide | - |
? | |
2.7.11.14 | ATP + protein | autophosphorylation | Bos taurus | ADP + phosphoprotein | - |
? | |
2.7.11.14 | ATP + rhodopsin | highly specific for photobleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | high-gain phosphorylation of rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | domain structure | Bos taurus | ADP + phosphorhodopsin | - |
? | |
2.7.11.14 | ATP + rhodopsin | second messenger-independent protein kinase, involved in the deactivation of photolyzed rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.14 | ? | x * 66000, autophosphorylated RK, SDS-PAGE | Bos taurus |
2.7.11.14 | ? | x * 65000, dephosphorylated RK, SDS-PAGE | Bos taurus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.14 | rhodopsin kinase | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.14 | 30 | - |
assay at | Bos taurus |
2.7.11.14 | 30 | - |
peptide assay | Bos taurus |