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Literature summary extracted from

  • Hoshi, M.; Nishida, E.; Sakai, H.
    Characterization of a mitogen-activated, Ca2+-sensitive microtubule-associated protein-2 kinase (1989), Eur. J. Biochem., 184, 477-486.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.17 Ca2+ reversible, kinetics Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.17 0.0016
-
 microtubule associated protein 2 epidermal growth factor activated cells Rattus norvegicus
2.7.11.17 0.03
-
 ATP epidermal growth factor activated cells Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.17 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.17 40000
-
 rat, growth-factor activated fibroblasts, gel filtration, sucrose density gradient centrifugation Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 partial: growth-factor stimulated 3Y1-cells Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 fibroblast 1HIRc B cells (overexpressing the human insulin receptor), embryonic cell line 3Y1, both with elevated kinase activity after treatment with mitogens, e.g. insulin or epidermal growth factor Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + histone 3S Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + histone 8S Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + microtubule associated protein 2
-
 Rattus norvegicus ADP + phosphorylated microtubule associated protein 2
-
 ?
2.7.11.17 ATP + microtubule-associated protein 2 as good as myelin basic protein Rattus norvegicus ADP + microtubule-associated protein 2 phosphate
-
 ?
2.7.11.17 ATP + microtubule-associated protein 2 phosphorylation sites are serine and threonine Rattus norvegicus ADP + microtubule-associated protein 2 phosphate
-
 ?
2.7.11.17 ATP + myelin basic protein phosphorylation sites: serine Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + myelin basic protein phosphorylation sites: threonine Rattus norvegicus ?
-
 ?
2.7.11.17 additional information phosphorylase b Rattus norvegicus ?
-
 ?
2.7.11.17 additional information no substrates are protamine Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 25
-
 assay at Rattus norvegicus