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Literature summary extracted from

  • Holtz, J.; Klämbt, D.
    tRNA isopentenyltransferase from Lactobacillus acidophilus ATCC 4963 (1975), Hoppe-Seyler's Z. Physiol. Chem., 356, 1459-1464.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.75 0.003
-
 DELTA2-isopentenyl diphosphate
-
 Lactobacillus acidophilus
2.5.1.75 0.013
-
 adenosine 37 in tRNA from Lactobacillus Lactobacillus acidophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.75 Mg2+ required Lactobacillus acidophilus
2.5.1.75 Mg2+ 5-7 mM Lactobacillus acidophilus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.75 Lactobacillus acidophilus
-
 ATCC 4963
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.75 partial Lactobacillus acidophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.75 additional information
-
-
 Lactobacillus acidophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA
-
 Lactobacillus acidophilus diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenosine 37 in tRNA
-
 Lactobacillus acidophilus N6-(dimethylallyl)adenosine37 in tRNA + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.75 7.5 8
-
 Lactobacillus acidophilus