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Literature summary extracted from

  • Rosenbaum, N.; Gefter, M.L.
    DELTA 2 -isopentenylpyrophosphate: transfer ribonucleic acid 2 -isopentenyltransferase from Escherichia coli. Purification and properties of the enzyme (1972), J. Biol. Chem., 247, 5675-5680.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.5.1.75 not stable to ammonium sulfate precipitation Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.75 diphosphate
-
 Escherichia coli
2.5.1.75 EDTA
-
 Escherichia coli
2.5.1.75 high ionic strength
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.75 Mg2+ required Escherichia coli
2.5.1.75 Mg2+ 3.3 mM Escherichia coli
2.5.1.75 Mn2+ can partially replace Mg2+ in activation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.75 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.75
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.75 0.053
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.5.1.75 0°C, stable for 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA tRNA lacking the isopentenyl modification normally present in vivo Escherichia coli diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA specific for DELTA2-isopentenyl phosphate Escherichia coli diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA no reaction with DELTA3-isopentenyl diphosphate Escherichia coli diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.75 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.75 6 9 about 50% of maximum activity at pH 6 and pH 9, below pH 5 activity is totally abolished Escherichia coli