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Literature summary extracted from

  • Juknat, A.A.; Doernemann, D.; Senger, H.
    Purification and kinetic studies on a porphobilinogen deaminase from the unicellular green alga Scenedesmus obliquus (1994), Planta, 193, 123-130.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.079
-
porphobilinogen 37°C, pH 7.4 Tetradesmus obliquus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 33000
-
gel filtration Tetradesmus obliquus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Tetradesmus obliquus
-
?
-
?
2.5.1.61 porphobilinogen Escherichia coli
-
?
-
?
2.5.1.61 porphobilinogen Homo sapiens
-
?
-
?
2.5.1.61 porphobilinogen Rattus norvegicus
-
?
-
?
2.5.1.61 porphobilinogen Saccharomyces cerevisiae
-
?
-
?
2.5.1.61 porphobilinogen Euglena gracilis
-
?
-
?
2.5.1.61 porphobilinogen Cereibacter sphaeroides
-
?
-
?
2.5.1.61 porphobilinogen Spinacia oleracea
-
?
-
?
2.5.1.61 porphobilinogen Pisum sativum
-
?
-
?
2.5.1.61 porphobilinogen Chlorella regularis
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Cereibacter sphaeroides
-
-
-
2.5.1.61 Chlorella regularis
-
-
-
2.5.1.61 Escherichia coli
-
-
-
2.5.1.61 Euglena gracilis
-
-
-
2.5.1.61 Homo sapiens
-
-
-
2.5.1.61 Pisum sativum
-
pea
-
2.5.1.61 Rattus norvegicus
-
-
-
2.5.1.61 Saccharomyces cerevisiae
-
-
-
2.5.1.61 Spinacia oleracea
-
spinach
-
2.5.1.61 Tetradesmus obliquus
-
wild-type D3, Sammlung von Algenkulturen, Pflanzenphysiologisches Institiut Universit„t G”ttingen, Germany
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61
-
Tetradesmus obliquus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 0.113
-
at pH 7.4 Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Tetradesmus obliquus uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Escherichia coli uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Homo sapiens uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Rattus norvegicus uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Saccharomyces cerevisiae uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Euglena gracilis uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Spinacia oleracea uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Pisum sativum uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Chlorella regularis uroporphyrinogen III + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Tetradesmus obliquus hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Escherichia coli hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Homo sapiens hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Rattus norvegicus hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Saccharomyces cerevisiae hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Euglena gracilis hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Spinacia oleracea hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Pisum sativum hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 4 porphobilinogen + H2O
-
Chlorella regularis hydroxylmethylbilane + 4 NH3
-
?
2.5.1.61 porphobilinogen
-
Tetradesmus obliquus ?
-
?
2.5.1.61 porphobilinogen
-
Escherichia coli ?
-
?
2.5.1.61 porphobilinogen
-
Homo sapiens ?
-
?
2.5.1.61 porphobilinogen
-
Rattus norvegicus ?
-
?
2.5.1.61 porphobilinogen
-
Saccharomyces cerevisiae ?
-
?
2.5.1.61 porphobilinogen
-
Euglena gracilis ?
-
?
2.5.1.61 porphobilinogen
-
Cereibacter sphaeroides ?
-
?
2.5.1.61 porphobilinogen
-
Spinacia oleracea ?
-
?
2.5.1.61 porphobilinogen
-
Pisum sativum ?
-
?
2.5.1.61 porphobilinogen
-
Chlorella regularis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 PBG-D
-
Tetradesmus obliquus
2.5.1.61 PBG-D
-
Rattus norvegicus
2.5.1.61 PBG-D
-
Saccharomyces cerevisiae
2.5.1.61 porphobilinogen deaminase
-
Tetradesmus obliquus
2.5.1.61 porphobilinogen deaminase
-
Escherichia coli
2.5.1.61 porphobilinogen deaminase
-
Homo sapiens
2.5.1.61 porphobilinogen deaminase
-
Rattus norvegicus
2.5.1.61 porphobilinogen deaminase
-
Euglena gracilis
2.5.1.61 porphobilinogen deaminase
-
Cereibacter sphaeroides
2.5.1.61 porphobilinogen deaminase
-
Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 additional information
-
-
Tetradesmus obliquus
2.5.1.61 additional information
-
-
Homo sapiens
2.5.1.61 6.9
-
-
Tetradesmus obliquus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 additional information
-
-
Tetradesmus obliquus
2.5.1.61 additional information
-
-
Escherichia coli
2.5.1.61 additional information
-
-
Euglena gracilis
2.5.1.61 6.3 7.5 pH 6.3 and pH 7.5: 85% of maximal activity Tetradesmus obliquus