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Literature summary extracted from

  • Haedener, A.; Matzinger, P.K.; Malashkevich, V.N.; Louie, G.V.; Wood, S.P.; Oliver, P.; Alefounder, P.R.; Pitt, A.R.; Abell, C.; Battersby, A.R.
    Purification, characterization, crystallisation and X-ray analysis of selenomethionine-labelled hydroxymethylbilane synthase from Escherichia coli (1993), Eur. J. Biochem., 211, 615-624.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.61 SeMet-labelled enzyme Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.61 SeMet-labelled enzyme Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.61 additional information all, six, methionine residues are replaced by SeMet Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 34268
-
 1 * 34268, calculation from sequence of amino acid Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Escherichia coli
-
 K12, mutant PO1562
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61 SeMet-labelled enzyme, i.e. [SeMet] HMBS, and wild-type enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Escherichia coli uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Escherichia coli hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 porphobilinogen
-
 Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.61 monomer 1 * 34268, calculation from sequence of amino acid Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 HMBS
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 7.4
-
 SeMet-labelled enzyme Escherichia coli