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Literature summary extracted from

  • Spano, A.J.; Timko, M.P.
    Isolation, characterization and partial amino acid sequence of a chloroplast-localized porphobilinogen deaminase from pea (Pisum sativum L.) (1991), Biochim. Biophys. Acta, 1076, 29-36.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 Ca2+ CaCl2, weak Pisum sativum
2.5.1.61 Co2+ CoCl2, strong Pisum sativum
2.5.1.61 Fe2+ FeSO4, strong Pisum sativum
2.5.1.61 Mg2+ MgCl2, weak Pisum sativum
2.5.1.61 Mn2+ MnCl2; strong inhibition at submillimolar concentrations Pisum sativum
2.5.1.61 Na+ NaCl greater than 300 mM Pisum sativum
2.5.1.61 NH4+
-
 Pisum sativum
2.5.1.61 Zn2+ ZnCl2, strong Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.61 chloroplast
-
 Pisum sativum 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 45000
-
 1 * 45000, SDS-PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Pisum sativum
-
 ?
-
 ?
2.5.1.61 porphobilinogen Glycine max
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Glycine max
-
 soya-bean
-
2.5.1.61 Pisum sativum
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61 dye-ligand affinity chromatography Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 callus
-
 Glycine max
-
2.5.1.61 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 0.0813
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
2.5.1.61 -20°C or 4°C, unstable, loss of activity after several days Pisum sativum
2.5.1.61 -20°C, crude plastide lysate is stable for at least 2 weeks Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Pisum sativum uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Glycine max uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Pisum sativum hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Glycine max hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 porphobilinogen
-
 Pisum sativum ?
-
 ?
2.5.1.61 porphobilinogen
-
 Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.61 monomer 1 * 45000, SDS-PAGE Pisum sativum

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 porphobilinogen deaminase
-
 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 7.9 8.2
-
 Pisum sativum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6.5 9 pH 6.5: 25% of maximal activity, pH 9.0: 50% of maximal activity Pisum sativum