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Literature summary extracted from

  • Shioi, Y.; Nagamine, M.; Kuroki, M.; Sasa, T.
    Purification by affinity chromatography and properties of uroporphyrinogen I synthetase from Chlorella regularis (1980), Biochim. Biophys. Acta, 616, 300-309.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 N-ethylmaleimide
-
 Cereibacter sphaeroides
2.5.1.61 N-ethylmaleimide 1 mM, 59% inhibition Chlorella regularis
2.5.1.61 N-ethylmaleimide
-
 Triticum aestivum
2.5.1.61 p-chloromercuribenzoate
-
 Cereibacter sphaeroides
2.5.1.61 p-chloromercuribenzoate 0.025 mM, 62% inhibition Chlorella regularis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.05
-
 porphobilinogen
-
 Triticum aestivum
2.5.1.61 0.072
-
 porphobilinogen
-
 Spinacia oleracea
2.5.1.61 0.085
-
 Hydroxymethylbilane
-
 Chlorella regularis
2.5.1.61 0.089
-
 porphobilinogen
-
 Chlorella regularis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 33000
-
 1 * 33000, calculation from amino acid residues Chlorella regularis
2.5.1.61 35000 36000 gel filtration Chlorella regularis
2.5.1.61 38000
-
 sucrose density gradient centrifugation Spinacia oleracea
2.5.1.61 40000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Triticum aestivum
-
 ?
-
 ?
2.5.1.61 porphobilinogen Cereibacter sphaeroides
-
 ?
-
 ?
2.5.1.61 porphobilinogen Spinacia oleracea
-
 ?
-
 ?
2.5.1.61 porphobilinogen Chlorella regularis
-
 ?
-
 ?
2.5.1.61 porphobilinogen Chlorella regularis S-50
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Cereibacter sphaeroides
-
-
-
2.5.1.61 Chlorella regularis
-
 S-50
-
2.5.1.61 Chlorella regularis S-50
-
 S-50
-
2.5.1.61 Spinacia oleracea
-
 spinach
-
2.5.1.61 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61
-
 Chlorella regularis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 germ
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 0.0058
-
-
 Chlorella regularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Triticum aestivum uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Spinacia oleracea uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Chlorella regularis uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Chlorella regularis S-50 uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Triticum aestivum hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Spinacia oleracea hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
2.5.1.61 4 porphobilinogen + H2O
-
 Chlorella regularis hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
2.5.1.61 4 porphobilinogen + H2O
-
 Chlorella regularis S-50 hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
2.5.1.61 porphobilinogen
-
 Triticum aestivum ?
-
 ?
2.5.1.61 porphobilinogen
-
 Cereibacter sphaeroides ?
-
 ?
2.5.1.61 porphobilinogen
-
 Spinacia oleracea ?
-
 ?
2.5.1.61 porphobilinogen
-
 Chlorella regularis ?
-
 ?
2.5.1.61 porphobilinogen
-
 Chlorella regularis S-50 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.61 monomer
-
 Cereibacter sphaeroides
2.5.1.61 monomer 1 * 33000, calculation from amino acid residues Chlorella regularis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 uroporphyrinogen I synthetase
-
 Cereibacter sphaeroides
2.5.1.61 uroporphyrinogen I synthetase
-
 Spinacia oleracea
2.5.1.61 uroporphyrinogen I synthetase
-
 Chlorella regularis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.61 65
-
 24% above the activity at 37°C Chlorella regularis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.61 20 80 10% of maximal activity at 20°C, 8% of maximal activity at 80°C Chlorella regularis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.61 65
-
 1 h, little loss of activity Chlorella regularis
2.5.1.61 75
-
 13% loss of activity after 1h Chlorella regularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 7.4
-
 both in phosphate buffer and Tris buffer Chlorella regularis
2.5.1.61 7.8 8.2
-
 Cereibacter sphaeroides
2.5.1.61 8.2
-
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6 9
-
 Chlorella regularis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.61 6 9 stable between Chlorella regularis