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Literature summary extracted from

  • Okada, S.; Devarenne, T.P.; Chappell, J.
    Molecular characterization of squalene synthase from the green microalga Botryococcus braunii, race B (2000), Arch. Biochem. Biophys., 373, 307-317.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.96 expressed in Escherichia coli BL21(DE3) cells Botryococcus braunii
1.3.1.97 expressed in Escherichia coli BL21(DE3) cells Botryococcus braunii
2.5.1.21
-
 Botryococcus braunii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.96 microsome
-
 Botryococcus braunii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.96 52500
-
 x * 52500, calculated from amino acid sequence and estimated from SDS-PAGE Botryococcus braunii
1.3.1.97 52500
-
 x * 52500, estimated from amino acid sequence and SDS-PAGE Botryococcus braunii
2.5.1.21 52500
-
 x * 52500, deduced from gene sequence Botryococcus braunii

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.96 Botryococcus braunii
-
 race B
-
1.3.1.96 Botryococcus braunii Berkeley
-
 race B
-
1.3.1.97 Botryococcus braunii
-
-
-
2.5.1.21 Botryococcus braunii Q9SDW9 green microalga, race B
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.96 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii squalene + diphosphate + NADP+
-
 ?
1.3.1.96 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii Berkeley squalene + diphosphate + NADP+
-
 ?
1.3.1.97 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii C30 botryococcene + NADP+ + diphosphate
-
 ?
1.3.1.97 presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii Berkeley C30 botryococcene + NADP+ + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.96 ? x * 52500, calculated from amino acid sequence and estimated from SDS-PAGE Botryococcus braunii
1.3.1.97 ? x * 52500, estimated from amino acid sequence and SDS-PAGE Botryococcus braunii
2.5.1.21 ? x * 52500, deduced from gene sequence Botryococcus braunii

Synonyms

EC Number Synonyms Comment Organism
1.3.1.96 squalene synthase
-
 Botryococcus braunii
1.3.1.97 BSS
-
 Botryococcus braunii

Expression

EC Number Organism Comment Expression
1.3.1.97 Botryococcus braunii gene expression is preferential during rapid growth up