EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.21 | yeast enzyme overexpressed in Escherichia coli | Saccharomyces cerevisiae |
EC Number | General Stability | Organism |
---|---|---|
2.5.1.21 | a combination of 10% methanol, 10% glycerol, 30 mM octyl-beta-D-glucopyranoside, 0.4% Brij-58, and 1 mM DTT in 25 mM sodium phosphate, pH 7.4, essential for stability and maximal activity | Saccharomyces cerevisiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.21 | 0.0025 | - |
farnesyl diphosphate | - |
Saccharomyces cerevisiae | |
2.5.1.21 | 0.5 | - |
NADPH | - |
Saccharomyces cerevisiae | |
2.5.1.21 | 3.6 | - |
NADH | - |
Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.21 | farnesyl diphosphate + farnesyl diphosphate | Saccharomyces cerevisiae | essential enzyme in cholesterol biosynthetic pathway | diphosphate + presqualene diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.21 | Saccharomyces cerevisiae | - |
overexpressed in Escherichia coli | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.21 | overexpressed in E. coli | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.21 | additional information | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.21 | (2E,6E)-farnesyl diphosphate + NADH + H+ | - |
Saccharomyces cerevisiae | squalene + diphosphate + NAD+ | - |
? | |
2.5.1.21 | farnesyl diphosphate + farnesyl diphosphate | - |
Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
2.5.1.21 | farnesyl diphosphate + farnesyl diphosphate | essential enzyme in cholesterol biosynthetic pathway | Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
2.5.1.21 | presqualene diphosphate + NAD(P)H | - |
Saccharomyces cerevisiae | squalene + NAD(P)+ + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.21 | ? | x * 42000-44000, enzyme expressed in Escherichia coli, SDS-PAGE, carboxy-terminal truncated enzyme | Saccharomyces cerevisiae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.21 | 0.53 | - |
farnesyl diphosphate | cosubstrate + NAD(P)H | Saccharomyces cerevisiae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.21 | NADH | preference for NADPH over NADH | Saccharomyces cerevisiae | |
2.5.1.21 | NADPH | preference for NADPH over NADH | Saccharomyces cerevisiae |