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Literature summary extracted from

  • LoGrasso, P.V.; Soltis, D.A.; Boettcher, B.R.
    Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase (1993), Arch. Biochem. Biophys., 307, 193-199.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.21 yeast enzyme overexpressed in Escherichia coli Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.5.1.21 a combination of 10% methanol, 10% glycerol, 30 mM octyl-beta-D-glucopyranoside, 0.4% Brij-58, and 1 mM DTT in 25 mM sodium phosphate, pH 7.4, essential for stability and maximal activity Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.21 0.0025
-
farnesyl diphosphate
-
Saccharomyces cerevisiae
2.5.1.21 0.5
-
NADPH
-
Saccharomyces cerevisiae
2.5.1.21 3.6
-
NADH
-
Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.21 farnesyl diphosphate + farnesyl diphosphate Saccharomyces cerevisiae essential enzyme in cholesterol biosynthetic pathway diphosphate + presqualene diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.21 Saccharomyces cerevisiae
-
overexpressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.21 overexpressed in E. coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.21 additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.21 (2E,6E)-farnesyl diphosphate + NADH + H+
-
Saccharomyces cerevisiae squalene + diphosphate + NAD+
-
?
2.5.1.21 farnesyl diphosphate + farnesyl diphosphate
-
Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
2.5.1.21 farnesyl diphosphate + farnesyl diphosphate essential enzyme in cholesterol biosynthetic pathway Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
?
2.5.1.21 presqualene diphosphate + NAD(P)H
-
Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.21 ? x * 42000-44000, enzyme expressed in Escherichia coli, SDS-PAGE, carboxy-terminal truncated enzyme Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.21 0.53
-
farnesyl diphosphate cosubstrate + NAD(P)H Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.21 NADH preference for NADPH over NADH Saccharomyces cerevisiae
2.5.1.21 NADPH preference for NADPH over NADH Saccharomyces cerevisiae