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Literature summary extracted from

  • Zhang, D.; Jennnings, S.M.; Robinson, G.W.; Poulter, C.D.
    Yeast squalene synthase: expression, purification, and characterization of soluble recombinant enzyme [published erratum appears in Arch Biochem Biophys 1993 Sep;305(2):622] (1993), Arch. Biochem. Biophys., 304, 133-143.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.5.1.21 bovine serum albumin activates recombinant enzyme Saccharomyces cerevisiae
2.5.1.21 Tween 20 stimulates recombinant enzyme Saccharomyces cerevisiae
2.5.1.21 Tween 40 stimulates recombinant enzyme Saccharomyces cerevisiae
2.5.1.21 Tween 80 stimulates recombinant enzyme Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.21 development of a plasmid for heterologous expression of yeast squalene synthase in Escherichia coli Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.5.1.21 methanol, 10%, does not stabilize Saccharomyces cerevisiae
2.5.1.21 octylglucoside, 5 mM, causes rapid loss of activity Saccharomyces cerevisiae
2.5.1.21 stable in buffer containing 5 mM octylglucoside and 15 mM CHAPS Saccharomyces cerevisiae
2.5.1.21 sucrose, 10%, does not stabilize Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.21 N-ethylmaleimide
-
 Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.21 Mg2+ required for squalene synthase reaction Saccharomyces cerevisiae
2.5.1.21 Mg2+ Mn2+ is more effective than Mg2+ in activation of dehydrosqualene formation Saccharomyces cerevisiae
2.5.1.21 Mn2+ stimulates, maximum stimulation at 0.1 mM Saccharomyces cerevisiae
2.5.1.21 Mn2+ more effective than Mg2+ in activation of dehydrosqualene formation Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.21 44000
-
 x * 44000, expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.21 farnesyl diphosphate + farnesyl diphosphate Saccharomyces cerevisiae first pathway-specific enzyme in cholesterol biosynthesis diphosphate + presqualene diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.21 Saccharomyces cerevisiae
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.21 expressed in E. coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.21 additional information
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.5.1.21 -70°C, stable Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.21 farnesyl diphosphate + farnesyl diphosphate
-
 Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
 ?
2.5.1.21 farnesyl diphosphate + farnesyl diphosphate first pathway-specific enzyme in cholesterol biosynthesis Saccharomyces cerevisiae diphosphate + presqualene diphosphate
-
 ?
2.5.1.21 presqualene diphosphate + NAD(P)H
-
 Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.21 ? x * 44000, expressed in Escherichia coli, SDS-PAGE Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.21 3.3
-
 farnesyl diphosphate in presence of 1% Tween 80 and Mg2+ Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.21 7
-
-
 Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.21 5.2 8.2 pH 5.2: about 45% of maximum activity, pH 8.2: about 75% of maximum activity Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.21 8.5
-
 irreversible denaturation above Saccharomyces cerevisiae