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Literature summary extracted from

  • Takatsuji, H.; Nishino, T.; Izui, K.; Katsuki, H.
    Formation of dehydrosqualene catalyzed by squalene synthetase in Saccharomyces cerevisiae (1982), J. Biochem., 91, 911-921.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.21 microsome
-
 Sus scrofa
-
-
2.5.1.21 microsome
-
 Saccharomyces cerevisiae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.21 Mg2+ no effect Sus scrofa
2.5.1.21 Mg2+ Mn2+ is six times more effective in activation than Mg2+ Saccharomyces cerevisiae
2.5.1.21 Mn2+ stimulation Sus scrofa
2.5.1.21 Mn2+ six times more effective in activation than Mg2+ Saccharomyces cerevisiae
2.5.1.21 Mn2+ in presence of Mn2+ and Mg2+ the activity is reduced to one-third of that in presence of Mn2+ alone Sus scrofa
2.5.1.21 Mn2+ in presence of Mn2+ and Mg2+ the activity is reduced to one-third of that in presence of Mn2+ alone Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.21 68000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.21 Saccharomyces cerevisiae
-
-
-
2.5.1.21 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.21 partial Saccharomyces cerevisiae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.21 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.21 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.21 farnesyl diphosphate
-
 Sus scrofa 12,13-cis-dehydrosqualene + diphosphate
-
 ?
2.5.1.21 farnesyl diphosphate
-
 Saccharomyces cerevisiae 12,13-cis-dehydrosqualene + diphosphate
-
 ?
2.5.1.21 farnesyl diphosphate + NAD(P)H
-
 Sus scrofa squalene + diphosphate + NAD(P)+
-
 ?
2.5.1.21 farnesyl diphosphate + NAD(P)H
-
 Saccharomyces cerevisiae squalene + diphosphate + NAD(P)+
-
 ?
2.5.1.21 presqualene diphosphate + NAD(P)H in presence of reducing pyridine nucleotide, preferably NADPH, squalene is formed, in absence of reducing cofactor the rate of the condensation reaction is lower and all of the product accumulates as presqualene diphosphate Sus scrofa squalene + NAD(P)+ + diphosphate
-
 ?
2.5.1.21 presqualene diphosphate + NAD(P)H in presence of reducing pyridine nucleotide, preferably NADPH, squalene is formed, in absence of reducing cofactor the rate of the condensation reaction is lower and all of the product accumulates as presqualene diphosphate Saccharomyces cerevisiae squalene + NAD(P)+ + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.21 30
-
 assay at Sus scrofa
2.5.1.21 30
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.21 NADPH
-
 Sus scrofa
2.5.1.21 NADPH
-
 Saccharomyces cerevisiae