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Literature summary extracted from
- Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities (1987), J. Biol. Chem., 262, 1505-1509.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.5.1.21 | methanol stabilizes | Saccharomyces cerevisiae |
| 2.5.1.21 | sucrose stabilizes | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.5.1.21 | microsome | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.5.1.21 | Mg2+ | required for squalene synthase reaction | Saccharomyces cerevisiae |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.21 | 55000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.5.1.21 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.5.1.21 | partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.5.1.21 | 0.95 | - |
squalene | Saccharomyces cerevisiae |
| 2.5.1.21 | 15.6 | - |
presqualene | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.5.1.21 | farnesyl diphosphate + farnesyl diphosphate | - |
Saccharomyces cerevisiae | diphosphate + presqualene diphosphate | - |
? | |
| 2.5.1.21 | presqualene diphosphate + NAD(P)H | presqualene diphosphate synthetase and squalene synthetase are copurified during isolation | Saccharomyces cerevisiae | squalene + NAD(P)+ + diphosphate | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.5.1.21 | NADH | - |
Saccharomyces cerevisiae | |
| 2.5.1.21 | NADPH | preference for NADPH over NADH | Saccharomyces cerevisiae | |
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