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Literature summary extracted from

  • Fu, H.A.; Hartman, A.; Lowery, R.G.; Fitzmaurice, W.P.; Roberts, G.P.; Burris, R.H.
    Posttranslational regulatory system for nitrogenase activity in Azospirillum spp (1989), J. Bacteriol., 171, 4679-4685.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.24 membrane
-
 Azospirillum brasilense 16020
-
3.2.2.24 membrane
-
 Azospirillum lipoferum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.37 Mg2+ activation Azospirillum brasilense

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex Azospirillum brasilense
-
 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex Azospirillum lipoferum
-
 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex Azospirillum brasilense Sp7
-
 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex Azospirillum lipoferum SpBr17
-
 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.37 Azospirillum brasilense
-
-
-
2.4.2.37 Azospirillum brasilense Sp7
-
-
-
2.4.2.37 Rhodospirillum rubrum
-
-
-
3.2.2.24 Azospirillum brasilense
-
 wild-type (Sp7)
-
3.2.2.24 Azospirillum lipoferum
-
 SpBr17
-
3.2.2.24 Azospirillum lipoferum SpBr17
-
 SpBr17
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.24
-
 Azospirillum lipoferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase]
-
 Azospirillum brasilense nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 ?
2.4.2.37 NAD+ + [dinitrogen reductase]
-
 Rhodospirillum rubrum nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 ?
2.4.2.37 NAD+ + [dinitrogen reductase]
-
 Azospirillum brasilense Sp7 nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex
-
 Azospirillum brasilense ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex
-
 Azospirillum lipoferum ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex
-
 Azospirillum brasilense Sp7 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?
3.2.2.24 ADP-D-ribosyl-[dinitrogen reductase] component of nitrogenase complex
-
 Azospirillum lipoferum SpBr17 ADPribose + [dinitrogen reductase] component of nitrogenase complex
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.37 30
-
 assay at Azospirillum brasilense
2.4.2.37 30
-
 assay at Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.2.37 ADP activation Azospirillum brasilense