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Literature summary extracted from

  • Lowery, R.G.; Ludden, P.W.
    Effect of nucleotides on the activity of dinitrogenase reductase ADP-ribosyltransferase from Rhodospirillum rubrum (1989), Biochemistry, 28, 4956-4961.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.37 2'-deoxy-ADP activation Rhodospirillum rubrum
2.4.2.37 ADP-beta-S i.e. adenosine 5'-O-(2-thiodiphosphate), activation Rhodospirillum rubrum
2.4.2.37 MgADP- stimulation by binding to dinitrogen reductase (not from Azotobacter vinelandii), no activation by ATP, GDP, IDP, etheno-ADP, AMP-CH2-P, 8-bromo-ADP Rhodospirillum rubrum

General Stability

EC Number General Stability Organism
2.4.2.37 bovine serum albumin increases stability towards freeze thawing in the absence of ADP Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.37 ATP in the presence of ADP, 63% inhibition at 1,5 mM Rhodospirillum rubrum
2.4.2.37 MgATP2- in the presence of Mg-ADP Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.37 0.7
-
 NAD+ cosubstrate ADP-D-ribosyl-[dinitrogen reductase] from Azotobacter vinelandii, in the absence of MgADP- Rhodospirillum rubrum
2.4.2.37 2
-
 NAD+ cosubstrate native ADP-D-ribosyl-[dinitrogen reductase], in the presence of MgADP- Rhodospirillum rubrum
2.4.2.37 2.5
-
 NAD+ cosubstrate ADP-D-ribosyl-[dinitrogen reductase] from Klebsiella pneumoniae, in the absence of MgADP- Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.37 Rhodospirillum rubrum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.37 0.004 0.015
-
 Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase] high specificity for acceptor substrate, the only acceptor is dinitrogen reductase Rhodospirillum rubrum nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.37 30
-
 assay at Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
2.4.2.37 2'-deoxy-ADP activation Rhodospirillum rubrum