BRENDA - Enzyme Database show

Purification and properties of dinitrogenase reductase ADP-ribosyltransferase from the photosynthetic bacterium Rhodospirillum rubrum

Lowery, R.G.; Ludden, P.W.; J. Biol. Chem. 263, 16714-16719 (1988)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.2.37
ADP and NaCl stabilize during purification and storage
Rhodospirillum rubrum
2.4.2.37
desalting procedures during purification inactivate
Rhodospirillum rubrum
2.4.2.37
DTT stabilizes during purification
Rhodospirillum rubrum
2.4.2.37
extremely unstable in crude extract or partially purified preparation
Rhodospirillum rubrum
2.4.2.37
one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes
Rhodospirillum rubrum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.37
3-aminobenzamide
-
Rhodospirillum rubrum
2.4.2.37
KCl
reversible
Rhodospirillum rubrum
2.4.2.37
NaBr
strong
Rhodospirillum rubrum
2.4.2.37
NaCl
reversible
Rhodospirillum rubrum
2.4.2.37
NADH
-
Rhodospirillum rubrum
2.4.2.37
NADP+
-
Rhodospirillum rubrum
2.4.2.37
nicotinamide
-
Rhodospirillum rubrum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.37
2
-
NAD+
-
Rhodospirillum rubrum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.37
soluble
-
Rhodospirillum rubrum
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.37
29000
-
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
2.4.2.37
30900
-
gel filtration
Rhodospirillum rubrum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.37
NAD+ + [dinitrogen reductase]
Rhodospirillum rubrum
involved in regulation of nitrogenase EC 1.18.6.1 activity
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.37
Rhodospirillum rubrum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.37
to near homogeneity
Rhodospirillum rubrum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.37
0.077
-
-
Rhodospirillum rubrum
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.37
-80°C, in liquid N2
Rhodospirillum rubrum
2.4.2.37
0°C, in 0.2 M NaCl and 1 mM ADP, 18 h
Rhodospirillum rubrum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.37
etheno-NAD+ + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
2.4.2.37
NAD+ + [dinitrogen reductase]
high specificity for acceptor substrate, the only acceptor is dinitrogen reductase
489801
Rhodospirillum rubrum
nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
2.4.2.37
NAD+ + [dinitrogen reductase]
involved in regulation of nitrogenase EC 1.18.6.1 activity
489801
Rhodospirillum rubrum
?
-
-
-
?
2.4.2.37
nicotinamide guanine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
2.4.2.37
nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.2.37
monomer
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.37
30
-
assay at
Rhodospirillum rubrum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.37
7
-
-
Rhodospirillum rubrum
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.37
ADP and NaCl stabilize during purification and storage
Rhodospirillum rubrum
2.4.2.37
desalting procedures during purification inactivate
Rhodospirillum rubrum
2.4.2.37
DTT stabilizes during purification
Rhodospirillum rubrum
2.4.2.37
extremely unstable in crude extract or partially purified preparation
Rhodospirillum rubrum
2.4.2.37
one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes
Rhodospirillum rubrum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.37
3-aminobenzamide
-
Rhodospirillum rubrum
2.4.2.37
KCl
reversible
Rhodospirillum rubrum
2.4.2.37
NaBr
strong
Rhodospirillum rubrum
2.4.2.37
NaCl
reversible
Rhodospirillum rubrum
2.4.2.37
NADH
-
Rhodospirillum rubrum
2.4.2.37
NADP+
-
Rhodospirillum rubrum
2.4.2.37
nicotinamide
-
Rhodospirillum rubrum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.37
2
-
NAD+
-
Rhodospirillum rubrum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.37
soluble
-
Rhodospirillum rubrum
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.2.37
29000
-
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
2.4.2.37
30900
-
gel filtration
Rhodospirillum rubrum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.37
NAD+ + [dinitrogen reductase]
Rhodospirillum rubrum
involved in regulation of nitrogenase EC 1.18.6.1 activity
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.37
to near homogeneity
Rhodospirillum rubrum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.37
0.077
-
-
Rhodospirillum rubrum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.37
-80°C, in liquid N2
Rhodospirillum rubrum
2.4.2.37
0°C, in 0.2 M NaCl and 1 mM ADP, 18 h
Rhodospirillum rubrum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.37
etheno-NAD+ + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
2.4.2.37
NAD+ + [dinitrogen reductase]
high specificity for acceptor substrate, the only acceptor is dinitrogen reductase
489801
Rhodospirillum rubrum
nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
2.4.2.37
NAD+ + [dinitrogen reductase]
involved in regulation of nitrogenase EC 1.18.6.1 activity
489801
Rhodospirillum rubrum
?
-
-
-
?
2.4.2.37
nicotinamide guanine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
2.4.2.37
nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase]
-
489801
Rhodospirillum rubrum
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.2.37
monomer
1 * 29000, SDS-PAGE
Rhodospirillum rubrum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.37
30
-
assay at
Rhodospirillum rubrum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.37
7
-
-
Rhodospirillum rubrum