EC Number | General Stability | Organism |
---|---|---|
2.4.2.37 | ADP and NaCl stabilize during purification and storage | Rhodospirillum rubrum |
2.4.2.37 | desalting procedures during purification inactivate | Rhodospirillum rubrum |
2.4.2.37 | DTT stabilizes during purification | Rhodospirillum rubrum |
2.4.2.37 | extremely unstable in crude extract or partially purified preparation | Rhodospirillum rubrum |
2.4.2.37 | one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes | Rhodospirillum rubrum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.37 | 3-aminobenzamide | - |
Rhodospirillum rubrum | |
2.4.2.37 | KCl | reversible | Rhodospirillum rubrum | |
2.4.2.37 | NaBr | strong | Rhodospirillum rubrum | |
2.4.2.37 | NaCl | reversible | Rhodospirillum rubrum | |
2.4.2.37 | NADH | - |
Rhodospirillum rubrum | |
2.4.2.37 | NADP+ | - |
Rhodospirillum rubrum | |
2.4.2.37 | nicotinamide | - |
Rhodospirillum rubrum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.37 | 2 | - |
NAD+ | - |
Rhodospirillum rubrum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.2.37 | soluble | - |
Rhodospirillum rubrum | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.37 | 29000 | - |
1 * 29000, SDS-PAGE | Rhodospirillum rubrum |
2.4.2.37 | 30900 | - |
gel filtration | Rhodospirillum rubrum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.37 | NAD+ + [dinitrogen reductase] | Rhodospirillum rubrum | involved in regulation of nitrogenase EC 1.18.6.1 activity | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.37 | Rhodospirillum rubrum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.37 | to near homogeneity | Rhodospirillum rubrum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.37 | 0.077 | - |
- |
Rhodospirillum rubrum |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.37 | -80°C, in liquid N2 | Rhodospirillum rubrum |
2.4.2.37 | 0°C, in 0.2 M NaCl and 1 mM ADP, 18 h | Rhodospirillum rubrum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.37 | etheno-NAD+ + [dinitrogen reductase] | - |
Rhodospirillum rubrum | ? | - |
? | |
2.4.2.37 | NAD+ + [dinitrogen reductase] | high specificity for acceptor substrate, the only acceptor is dinitrogen reductase | Rhodospirillum rubrum | nicotinamide + ADP-D-ribosyl-[dinitrogen reductase] | - |
? | |
2.4.2.37 | NAD+ + [dinitrogen reductase] | involved in regulation of nitrogenase EC 1.18.6.1 activity | Rhodospirillum rubrum | ? | - |
? | |
2.4.2.37 | nicotinamide guanine dinucleotide + [dinitrogen reductase] | - |
Rhodospirillum rubrum | ? | - |
? | |
2.4.2.37 | nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase] | - |
Rhodospirillum rubrum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.37 | monomer | 1 * 29000, SDS-PAGE | Rhodospirillum rubrum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.37 | 30 | - |
assay at | Rhodospirillum rubrum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.37 | 7 | - |
- |
Rhodospirillum rubrum |