BRENDA - Enzyme Database show

The exotoxin P. aeruginosa: a proenzyme having an unusual mode of activation

Leppla, S.H.; Martin, O.C.; Muehl, L.A.; Biochem. Biophys. Res. Commun. 81, 532-538 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.36
2-mercaptoethanol
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
2.4.2.36
cysteine
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
2.4.2.36
dithiothreitol
enhances activity together with urea
Pseudomonas aeruginosa
2.4.2.36
guanidine hydrochloride
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
2.4.2.36
additional information
exotoxin is synthesized in a catalytically inactive, proenzyme form, unfolding of the toxin molecule may cause activation
Pseudomonas aeruginosa
2.4.2.36
SDS
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
2.4.2.36
sulfite
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
2.4.2.36
Urea
enhances activity together with dithiothreitol
Pseudomonas aeruginosa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.36
extracellular
exotoxin A
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
Pseudomonas aeruginosa
NAD-glycohydrolase activity without acceptor substrate
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.36
Pseudomonas aeruginosa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.36
culture supernatant
-
Pseudomonas aeruginosa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
NAD-glycohydrolase activity without acceptor substrate
489800
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489800
Pseudomonas aeruginosa
-
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.2.36
2-mercaptoethanol
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
2.4.2.36
cysteine
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
2.4.2.36
dithiothreitol
enhances activity together with urea
Pseudomonas aeruginosa
2.4.2.36
guanidine hydrochloride
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
2.4.2.36
additional information
exotoxin is synthesized in a catalytically inactive, proenzyme form, unfolding of the toxin molecule may cause activation
Pseudomonas aeruginosa
2.4.2.36
SDS
enhances activity together with dithiothreitol, cysteine, 2-mercaptoethanol or sulfite
Pseudomonas aeruginosa
2.4.2.36
sulfite
activation, together with SDS or guanidine hydrochloride
Pseudomonas aeruginosa
2.4.2.36
Urea
enhances activity together with dithiothreitol
Pseudomonas aeruginosa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.36
extracellular
exotoxin A
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
Pseudomonas aeruginosa
NAD-glycohydrolase activity without acceptor substrate
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.36
culture supernatant
-
Pseudomonas aeruginosa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
NAD-glycohydrolase activity without acceptor substrate
489800
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489800
Pseudomonas aeruginosa
-