BRENDA - Enzyme Database show

Cellular ADP-ribosyltransferase with the same mechanism of action as diphtheria toxin and Pseudomonas toxin A

Lee, H.; Iglewski, W.J.; Proc. Natl. Acad. Sci. USA 81, 2703-2707 (1984)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.36
Cytoplasmic extract of pyBHK-cells
not fragment A
Mesocricetus auratus
2.4.2.36
histamine
250 mM, almost complete inhibition
Mesocricetus auratus
2.4.2.36
additional information
cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum
Mesocricetus auratus
2.4.2.36
additional information
fragment A and toxin A are not inhibited by histamine
Pseudomonas aeruginosa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.36
extracellular
-
Bos taurus
-
-
2.4.2.36
extracellular
-
Mesocricetus auratus
-
-
2.4.2.36
extracellular
fragment A of diphtheria toxin
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
Pseudomonas aeruginosa
-
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
r
2.4.2.36
NAD+ + elongation factor 2
Bos taurus
liver enzyme
nicotinamide + ADPribose-elongation factor 2
-
Bos taurus
?
2.4.2.36
NAD+ + elongation factor 2
Mesocricetus auratus
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
Mesocricetus auratus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.36
Bos taurus
-
-
-
2.4.2.36
Mesocricetus auratus
-
baby
-
2.4.2.36
Pseudomonas aeruginosa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.36
kidney
polyoma virus transformed baby hamster kidney cells, i.e. pyBHK-cells
Mesocricetus auratus
-
2.4.2.36
liver
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
-
489798
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489798
Pseudomonas aeruginosa
r
2.4.2.36
NAD+ + elongation factor 2
liver enzyme
489798
Bos taurus
nicotinamide + ADPribose-elongation factor 2
-
489798
Bos taurus
?
2.4.2.36
NAD+ + elongation factor 2
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
489798
Mesocricetus auratus
nicotinamide + ADPribose-elongation factor 2
-
489798
Mesocricetus auratus
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.36
22
-
assay at
Mesocricetus auratus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.36
6.6
-
assay at, nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
2.4.2.36
8
-
assay at, NAD+ + elongation factor 2
Mesocricetus auratus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.36
Cytoplasmic extract of pyBHK-cells
not fragment A
Mesocricetus auratus
2.4.2.36
histamine
250 mM, almost complete inhibition
Mesocricetus auratus
2.4.2.36
additional information
cellular ADPribosyltransferase is not inhibited by anti-fragment A-antiserum
Mesocricetus auratus
2.4.2.36
additional information
fragment A and toxin A are not inhibited by histamine
Pseudomonas aeruginosa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.36
extracellular
-
Bos taurus
-
-
2.4.2.36
extracellular
-
Mesocricetus auratus
-
-
2.4.2.36
extracellular
fragment A of diphtheria toxin
Pseudomonas aeruginosa
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
Pseudomonas aeruginosa
-
nicotinamide + ADPribose-elongation factor 2
-
Pseudomonas aeruginosa
r
2.4.2.36
NAD+ + elongation factor 2
Bos taurus
liver enzyme
nicotinamide + ADPribose-elongation factor 2
-
Bos taurus
?
2.4.2.36
NAD+ + elongation factor 2
Mesocricetus auratus
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
Mesocricetus auratus
r
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.36
kidney
polyoma virus transformed baby hamster kidney cells, i.e. pyBHK-cells
Mesocricetus auratus
-
2.4.2.36
liver
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.36
NAD+ + elongation factor 2
-
489798
Pseudomonas aeruginosa
nicotinamide + ADPribose-elongation factor 2
-
489798
Pseudomonas aeruginosa
r
2.4.2.36
NAD+ + elongation factor 2
liver enzyme
489798
Bos taurus
nicotinamide + ADPribose-elongation factor 2
-
489798
Bos taurus
?
2.4.2.36
NAD+ + elongation factor 2
i.e. elongation factor 2 of baby hamster kidney cells, forward ADP-ribosylation reaction is reversed by fragment A from Pseudomonas aeruginosa
489798
Mesocricetus auratus
nicotinamide + ADPribose-elongation factor 2
-
489798
Mesocricetus auratus
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.36
22
-
assay at
Mesocricetus auratus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.36
6.6
-
assay at, nicotinamide + ADPribose-elongation factor 2
Mesocricetus auratus
2.4.2.36
8
-
assay at, NAD+ + elongation factor 2
Mesocricetus auratus