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Literature summary extracted from

  • Cameron, B.; Blanche, F.; Rouyez, M.C.; Bisch, D.; Famechon, A.; Couder, M.; Cauchois, L.; Thibaut, D.; Debussche, L.; Crouzet, J.
    Genetic analysis, nucleotide sequence, and products of two Pseudomonas denitrificans cob genes encoding nicotinate-nucleotide: dimethylbenzimidazole phosphoribosyltransferase and cobalamin (5-phosphate) synthase (1991), J. Bacteriol., 173, 6066-6073.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.21
-
 Pseudomonas denitrificans (nom. rej.)
2.7.8.26 expression of truncated CobV missing the first 20 codons in Escherichia coli Pseudomonas denitrificans (nom. rej.)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.21 0.000016
-
 dimethylbenzimidazole
-
 Pseudomonas denitrificans (nom. rej.)
2.4.2.21 0.083
-
 nicotinic acid mononucleotide
-
 Pseudomonas denitrificans (nom. rej.)
2.7.8.26 0.0009
-
 adenosylcobinamide-GDP 30°C, pH 9.0 Pseudomonas denitrificans (nom. rej.)
2.7.8.26 0.0027
-
 alpha-ribazole 5'-phosphate 30°C, pH 9.0 Pseudomonas denitrificans (nom. rej.)
2.7.8.26 0.0078
-
 alpha-ribazole 30°C, pH 9.0 Pseudomonas denitrificans (nom. rej.)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.21 35000
-
 2 * 35000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)
2.4.2.21 71000
-
 gel filtration Pseudomonas denitrificans (nom. rej.)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole Pseudomonas denitrificans (nom. rej.)
-
 GMP + adenosylcobalamin
-
 ?
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate Pseudomonas denitrificans (nom. rej.)
-
 GMP + adenosylcobalamin 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.21 Pseudomonas denitrificans (nom. rej.) P29935
-
-
2.7.8.26 Pseudomonas denitrificans (nom. rej.) P29936
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.21
-
 Pseudomonas denitrificans (nom. rej.)
2.7.8.26 Superose 12 HR, Phenyl-Superose, partially purified Pseudomonas denitrificans (nom. rej.)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.21 additional information
-
-
 Pseudomonas denitrificans (nom. rej.)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.21 beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole
-
 Pseudomonas denitrificans (nom. rej.) nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole
-
 ?
2.4.2.21 nicotinic acid mononucleotide + benzimidazole
-
 Pseudomonas denitrificans (nom. rej.) 1-alpha-D-ribofuranosylbenzimidazole 5'-phosphate + nicotinate alpha-ribazole 5'-phosphate ?
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole
-
 Pseudomonas denitrificans (nom. rej.) GMP + adenosylcobalamin
-
 ?
2.7.8.26 adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate
-
 Pseudomonas denitrificans (nom. rej.) GMP + adenosylcobalamin 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.21 dimer 2 * 35000, SDS-PAGE Pseudomonas denitrificans (nom. rej.)

Synonyms

EC Number Synonyms Comment Organism
2.7.8.26 CobV
-
 Pseudomonas denitrificans (nom. rej.)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.21 30
-
 assay at Pseudomonas denitrificans (nom. rej.)