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Literature summary extracted from

  • Yamada, E.W.
    The phosphorolysis of nucleosides by rabbit bone marrow (1961), J. Biol. Chem., 236, 3043-3046.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.15 2-mercaptoethanol required for optimal activity Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.15 PCMB 0.2 mM, complete inhibition Oryctolagus cuniculus
2.4.2.15 Tris buffer 0.1 M, pH 7.5, 22% inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.15 0.21
-
deoxyguanosine
-
Oryctolagus cuniculus
2.4.2.15 0.216
-
guanosine
-
Oryctolagus cuniculus
2.4.2.15 0.376
-
phosphate reaction with guanosine Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.15 guanosine + phosphate Oryctolagus cuniculus enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood guanine + D-ribose 1-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.15 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.15
-
Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.15 bone marrow
-
Oryctolagus cuniculus
-
2.4.2.15 intestinal mucosa
-
Oryctolagus cuniculus
-
2.4.2.15 liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.15 4.87
-
-
Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.4.2.15 -10 C, stable for 1 month Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.15 deoxyguanosine + phosphate
-
Oryctolagus cuniculus guanine + deoxyribose 1-phosphate
-
?
2.4.2.15 guanosine + phosphate equilibrium constant is 0.019 Oryctolagus cuniculus guanine + D-ribose 1-phosphate
-
r
2.4.2.15 guanosine + phosphate enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood Oryctolagus cuniculus guanine + D-ribose 1-phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.15 7
-
phosphorolysis of deoxyguanosine Oryctolagus cuniculus
2.4.2.15 7 7.4 phosphorolysis of guanosine Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.15 5 8.5 pH 5.0: about 35% of activity maximum, pH 8.5: about 45% of activity maximum Oryctolagus cuniculus