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Literature summary extracted from

  • Liras, A.; Argomaniz, L.; Llorente, P.
    Presence, preliminary properties and partial purification of 5-phosphoribosylpyrophosphate amidotransferase from Artemia sp (1990), Biochim. Biophys. Acta, 1033, 114-117.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.14 5',5'-P1,P4-diguanosine tetraphosphate IC50: 0.4 mM, 2 mM, approx. 80% inhibition Artemia sp.
2.4.2.14 ADP 1 mM, approx. 95% inhibition Artemia sp.
2.4.2.14 AMP 1 mM, approx. 40% inhibition Artemia sp.
2.4.2.14 GDP 1 mM, approx. 75% inhibition Artemia sp.
2.4.2.14 GMP 1 mM, approx. 60% inhibition Artemia sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.14 0.4
-
 5-phospho-alpha-D-ribose 1-diphosphate cosubstrate NH3 Artemia sp.
2.4.2.14 0.7
-
 5-phospho-alpha-D-ribose 1-diphosphate cosubstrate glutamine Artemia sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.14 soluble
-
 Artemia sp.
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.14 Artemia sp.
-
 brine shrimp, activity increases 7fold during early larval development in 2 d old Artemia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.14 partial Artemia sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.14 nauplius
-
 Artemia sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.14 L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
 Artemia sp. 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
 ir
2.4.2.14 NH3 + 5-phospho-alpha-D-ribose 1-diphosphate + H2O lower aminotransferase activity compared to amidotransferase activity Artemia sp. 5-phospho-beta-D-ribosylamine + diphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.14 37
-
 assay at Artemia sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.2.14 0.4
-
 IC50: 0.4 mM, 2 mM, approx. 80% inhibition Artemia sp. 5',5'-P1,P4-diguanosine tetraphosphate