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Literature summary extracted from

  • Caskey, C.T.; Ashton, D.M.; Wyngaarden, J.B.
    The enzymology of feedback inhibition of glutamine phosphoribosylpyrophosphate amidotransferase by purine ribonucleotides (1964), J. Biol. Chem., 239, 2570-2579.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.14 2-mercaptoethanol stabilizes during purification Columba livia
2.4.2.14 prolonged dialysis against distilled water leads to precipitation Columba livia

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.14 ADP
-
 Columba livia
2.4.2.14 AMP
-
 Columba livia
2.4.2.14 AMP
-
 Rattus norvegicus
2.4.2.14 ATP
-
 Columba livia
2.4.2.14 GDP
-
 Columba livia
2.4.2.14 GMP
-
 Columba livia
2.4.2.14 GMP
-
 Rattus norvegicus
2.4.2.14 IMP
-
 Columba livia
2.4.2.14 additional information not inhibited by ribose 5-phosphate, purine ribonucleosides or bases, 2'- or 3'-phosphate or deoxyribose phosphate analogues or by pyrimidine ribonucleotides Columba livia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.14 0.086
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rattus norvegicus
2.4.2.14 0.1
-
 L-glutamine
-
 Columba livia
2.4.2.14 0.24
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Columba livia
2.4.2.14 0.53
-
 L-glutamine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.14 soluble
-
 Rattus norvegicus
-
-
2.4.2.14 soluble
-
 Columba livia
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.14 Columba livia
-
 pigeon
-
2.4.2.14 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.14 ammonium sulfate, heat treatment, calcium phosphate gel, DEAE-cellulose Columba livia
2.4.2.14 partial Rattus norvegicus
2.4.2.14 partial Columba livia

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.14 liver
-
 Rattus norvegicus
-
2.4.2.14 liver
-
 Columba livia
-

Storage Stability

EC Number Storage Stability Organism
2.4.2.14 2-mercaptoethanol stabilizes during storage Columba livia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.14 L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
 Rattus norvegicus 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
 ?
2.4.2.14 L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
 Columba livia 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.14 60
-
 6 min, about 25% loss of activity Columba livia
2.4.2.14 60
-
 after 3-6 min loss of ATP-sensitivity Columba livia

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.14 0.031 1.1 ATP
-
 Columba livia
2.4.2.14 0.038 0.64 ADP
-
 Columba livia
2.4.2.14 0.086 0.35 GMP
-
 Columba livia
2.4.2.14 0.092 2.5 AMP
-
 Columba livia
2.4.2.14 0.18 3.5 IMP
-
 Columba livia
2.4.2.14 0.38 5.4 GDP
-
 Columba livia