BRENDA - Enzyme Database show

Determination of nicotinamide phosphoribosyltransferase activity in human erythrocytes: high-performance liquid chromatography-linked method

Rocchigiani, M.; Micheli, V.; Duley, J.A.; Simmonds, H.A.; Anal. Biochem. 205, 334-336 (1992)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.00127
-
nicotinamide
-
Homo sapiens
2.4.2.12
0.00537
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
activation, up to 10 mM
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
additional information
-
activity per g hemoglobin
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.12
-70°C, in crude erythrocyte lysates, at least 30 days
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489742
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489742
Homo sapiens
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
activation, up to 0.4 mM
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
activation, up to 0.4 mM
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.00127
-
nicotinamide
-
Homo sapiens
2.4.2.12
0.00537
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
activation, up to 10 mM
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
Homo sapiens
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
erythrocyte
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.2.12
additional information
-
activity per g hemoglobin
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.12
-70°C, in crude erythrocyte lysates, at least 30 days
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489742
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489742
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Homo sapiens