BRENDA - Enzyme Database show

Evidence for a physiologically active nicotinamide phosphoribosyltransferase in cultured human fibroblasts

Elliott, G.C.; Rechsteiner, M.C.; Biochem. Biophys. Res. Commun. 104, 996-1002 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.2.12
freeze-thawing inactivates
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
additional information
nicotinic acid, thymine, 5-bromouracil
Homo sapiens
2.4.2.12
NAD+
-
Homo sapiens
2.4.2.12
nicotinate adenine dinucleotide
not inhibitory
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.0016
-
nicotinamide
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
requirement
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Homo sapiens
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
fibroblast
diploid, GM 1362, from patient with Lesch-Nyhan disease
Homo sapiens
-
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.12
-80°C, crude, 4-6 months
Homo sapiens
2.4.2.12
4°C, crude, inactivation overnight
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489740
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489740
Homo sapiens
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
specific for nicotinamide, no substrates: thymine, 5-bromouracil, nicotinic acid adenine dinucleotide
489740
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489740
Homo sapiens
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
requirement
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.4.2.12
ATP
requirement
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.12
freeze-thawing inactivates
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
additional information
nicotinic acid, thymine, 5-bromouracil
Homo sapiens
2.4.2.12
NAD+
-
Homo sapiens
2.4.2.12
nicotinate adenine dinucleotide
not inhibitory
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
0.0016
-
nicotinamide
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
requirement
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
Homo sapiens
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
fibroblast
diploid, GM 1362, from patient with Lesch-Nyhan disease
Homo sapiens
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.12
-80°C, crude, 4-6 months
Homo sapiens
2.4.2.12
4°C, crude, inactivation overnight
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489740
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489740
Homo sapiens
?
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
specific for nicotinamide, no substrates: thymine, 5-bromouracil, nicotinic acid adenine dinucleotide
489740
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489740
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
37
-
assay at
Homo sapiens