BRENDA - Enzyme Database show

Enzymatic synthesis of nicotinamide mononucleotide

Preiss, J.; Handler, P.; J. Biol. Chem. 225, 759-770 (1957)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
hypoxanthine
phosphorolysis
Homo sapiens
2.4.2.12
additional information
no inhibition by NaF
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
100
-
nicotinamide
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
Km-value: 0.6 mM
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.12
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.12
partial
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
erythrocyte
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489737
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489737
Homo sapiens
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
35
-
assay at
Homo sapiens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.2.12
65
-
10 min, stable, heat treatment during purification
Homo sapiens
2.4.2.12
70
-
5 min, stable, heat treatment during purification
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.12
6.8
7.6
-
Homo sapiens
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.12
6.1
8.5
about half-maximal activity at pH 6.1 and pH 8.5
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.12
hypoxanthine
phosphorolysis
Homo sapiens
2.4.2.12
additional information
no inhibition by NaF
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.12
100
-
nicotinamide
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.12
soluble
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.12
Mg2+
Km-value: 0.6 mM
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
-
nicotinamide D-ribonucleotide + diphosphate
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.12
partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.12
erythrocyte
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.12
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
-
489737
Homo sapiens
nicotinamide D-ribonucleotide + diphosphate
-
489737
Homo sapiens
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.2.12
35
-
assay at
Homo sapiens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.4.2.12
65
-
10 min, stable, heat treatment during purification
Homo sapiens
2.4.2.12
70
-
5 min, stable, heat treatment during purification
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.12
6.8
7.6
-
Homo sapiens
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.2.12
6.1
8.5
about half-maximal activity at pH 6.1 and pH 8.5
Homo sapiens