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Literature summary extracted from

  • Victor, J.; Greenberg, L.B.; Sloan, D.L.
    Studies of the kinetic mechanism of orotate phosphoribosyltransferase from yeast (1979), J. Biol. Chem., 254, 2647-2655.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 5-phospho-alpha-D-ribose 1-diphosphate phosphorolysis, product inhibition, kinetics Saccharomyces cerevisiae
2.4.2.10 anthranilate weak Saccharomyces cerevisiae
2.4.2.10 arabinose 5-phosphate at higher concentrations Saccharomyces cerevisiae
2.4.2.10 diphosphate product inhibition, kinetics Saccharomyces cerevisiae
2.4.2.10 erythrose 4-phosphate at higher concentrations Saccharomyces cerevisiae
2.4.2.10 fructose 1-phosphate at higher concentrations Saccharomyces cerevisiae
2.4.2.10 fructose 6-phosphate at higher concentrations Saccharomyces cerevisiae
2.4.2.10 nicotinate weak Saccharomyces cerevisiae
2.4.2.10 Orotate phosphorolysis, product inhibition, kinetics Saccharomyces cerevisiae
2.4.2.10 orotidylate product inhibition, kinetics Saccharomyces cerevisiae
2.4.2.10 phosphate at higher concentrations Saccharomyces cerevisiae
2.4.2.10 ribose 5-phosphate at higher concentrations Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 additional information
-
 additional information kinetic study Saccharomyces cerevisiae
2.4.2.10 0.008 0.0083 orotidine 5'-phosphate phosphorolysis Saccharomyces cerevisiae
2.4.2.10 0.027 0.0275 Orotate
-
 Saccharomyces cerevisiae
2.4.2.10 0.03 0.035 Orotate
-
 Saccharomyces cerevisiae
2.4.2.10 0.038 0.04 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharomyces cerevisiae
2.4.2.10 0.096
-
 diphosphate phosphorolysis Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.10 Mg2+ requirement Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 20000
-
 2 * 20000, SDS-PAGE Saccharomyces cerevisiae
2.4.2.10 40000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Saccharomyces cerevisiae
-
 Budweiser brand
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.10 orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate mechanism is bi bi ping pong Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 45
-
 in the absence of added OMP-decarboxylase, EC 4.1.1.23 Saccharomyces cerevisiae
2.4.2.10 65
-
 in the presence of added OMP-decarboxylase, EC 4.1.1.23 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 5-fluoroorotate + phosphoribose diphosphate
-
 Saccharomyces cerevisiae 5-fluoroorotidine 5'-phosphate + diphosphate
-
 ?
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate catalyzes stereospecific formation of beta-glycosidic bond between orotate and ribose 5'-phosphate portion of phospho-ribose diphosphate Saccharomyces cerevisiae orotidine 5'-phosphate + diphosphate
-
 r
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate predominant species of phosphoribose diphosphate: metal ion complex Saccharomyces cerevisiae orotidine 5'-phosphate + diphosphate
-
 r
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate high specificity for orotate Saccharomyces cerevisiae orotidine 5'-phosphate + diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.10 dimer 2 * 20000, SDS-PAGE Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.10 25
-
 assay at Saccharomyces cerevisiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.10 0.01
-
 orotydilate
-
 Saccharomyces cerevisiae
2.4.2.10 0.042
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharomyces cerevisiae
2.4.2.10 0.063
-
 Orotate
-
 Saccharomyces cerevisiae
2.4.2.10 0.131
-
 diphosphate
-
 Saccharomyces cerevisiae