EC Number | General Stability | Organism |
---|---|---|
2.4.2.10 | 5 mM Mg2+, 1 mM phosphoribose diphosphate and 2 mM DTT stabilize dilute enzyme solutions | Mus musculus |
2.4.2.10 | dialysis against phosphate buffer, pH 7 or 7.5, unstable to, 2 mM DTT protect | Mus musculus |
2.4.2.10 | dilution inactivates | Mus musculus |
2.4.2.10 | phosphate, 0.3 M and above, stabilizes | Mus musculus |
2.4.2.10 | proteins, e.g. albumin, do not stabilize dilute enzyme solutions | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.10 | 5-Bromouracil | - |
Mus musculus | |
2.4.2.10 | 5-Chlorouracil | - |
Mus musculus | |
2.4.2.10 | 5-Fluoroorotate | at 0.05 M | Mus musculus | |
2.4.2.10 | adenine | - |
Mus musculus | |
2.4.2.10 | adenosine | - |
Mus musculus | |
2.4.2.10 | ADP | - |
Mus musculus | |
2.4.2.10 | allopurinol | - |
Mus musculus | |
2.4.2.10 | AMP | - |
Mus musculus | |
2.4.2.10 | ATP | - |
Mus musculus | |
2.4.2.10 | Azauracil | - |
Mus musculus | |
2.4.2.10 | Azauridine | - |
Mus musculus | |
2.4.2.10 | Barbituric acid | - |
Mus musculus | |
2.4.2.10 | cytosine | - |
Mus musculus | |
2.4.2.10 | dihydroorotate | 10 mM | Mus musculus | |
2.4.2.10 | diphosphate | - |
Mus musculus | |
2.4.2.10 | dUMP | - |
Mus musculus | |
2.4.2.10 | GMP | - |
Mus musculus | |
2.4.2.10 | IMP | - |
Mus musculus | |
2.4.2.10 | additional information | not inhibitory: CMP, TMP, 6-aza-UMP or 5-bromo-UMP | Mus musculus | |
2.4.2.10 | Orotidine | 10 mM | Mus musculus | |
2.4.2.10 | Oxipurinol | - |
Mus musculus | |
2.4.2.10 | phosphate | - |
Mus musculus | |
2.4.2.10 | ribose 5-phosphate | not inhibitory | Mus musculus | |
2.4.2.10 | UDP | - |
Mus musculus | |
2.4.2.10 | UMP | 1 mM, not 0.1 mM | Mus musculus | |
2.4.2.10 | Uracil | - |
Mus musculus | |
2.4.2.10 | uridine | - |
Mus musculus | |
2.4.2.10 | UTP | - |
Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.10 | additional information | - |
additional information | principles of assay | Mus musculus | |
2.4.2.10 | 0.002 | - |
Orotate | - |
Mus musculus | |
2.4.2.10 | 0.016 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.10 | Mg2+ | requirement | Mus musculus | |
2.4.2.10 | Mg2+ | Km-value: 3 mM | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.10 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.10 | - |
Mus musculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.2.10 | Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.2.10 | 0.115 | - |
- |
Mus musculus |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.2.10 | -60°C to-20°C, crude enzyme or ammonium sulfate preparation, 2 years | Mus musculus |
2.4.2.10 | frozen, partially purified enzyme in phosphate buffer, pH 7 or 7.5, 2 months | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.10 | orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus | orotidine 5'-phosphate + diphosphate | i.e. orotidylate or OMP | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.2.10 | More | constitutes together with orotidylate decarboxylase (EC 4.1.1.23) UMP-synthase, former complex U or multienzyme pyr-5,6 | Mus musculus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.2.10 | 37 | - |
assay at | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.10 | 7 | 7.5 | preferred buffer: Tris-HCl, when substrates are non-saturating, phosphate buffer, when substrate are saturating, not: maleate, imidazole, N-tris[hydroxymethyl] methyl-2-aminoethane-sulfonic acid (i.e. TES) or 3-[N-morpholino]-2-hydroxypropanesulfonic acid (i.e. MOPS) buffer | Mus musculus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.10 | 4 | 9 | stable | Mus musculus |