Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Erion, M.D.; Takabayashi, K.; Smith, H.B.; Kessi, J.; Wagner, S.; Hoenger, S.; Shames, S.L.; Ealick, S.E.
    Purine nucleoside phosphorylase. 1. Structure-function Studies (1997), Biochemistry, 36, 11725-11734.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 expression of site-directed mutants in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.1 H86A 10-25fold reduction in catalytic activity Homo sapiens
2.4.2.1 N243A 100fold decrease in turnover-number compared to wild-type enzyme Homo sapiens
2.4.2.1 Q89A 10-25fold reduction in catalytic activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.01
-
 hypoxanthine mutant enzyme Y88F Homo sapiens
2.4.2.1 0.013
-
 hypoxanthine mutant enzyme S33A Homo sapiens
2.4.2.1 0.019
-
 hypoxanthine mutant enzyme H86A Homo sapiens
2.4.2.1 0.02
-
 hypoxanthine mutant enzyme R84A and H257A Homo sapiens
2.4.2.1 0.022
-
 Inosine mutant enzymes N243A and T242A Homo sapiens
2.4.2.1 0.024
-
 hypoxanthine mutant enzyme T242A Homo sapiens
2.4.2.1 0.025
-
 hypoxanthine mutant enzyme F159A Homo sapiens
2.4.2.1 0.03
-
 hypoxanthine wild-type enzyme Homo sapiens
2.4.2.1 0.037
-
 Inosine mutant enzyme K244A Homo sapiens
2.4.2.1 0.04
-
 Inosine wild-type enzyme Homo sapiens
2.4.2.1 0.04
-
 hypoxanthine mutant enzyme K244A Homo sapiens
2.4.2.1 0.058
-
 Inosine mutant enzyme Y88F Homo sapiens
2.4.2.1 0.13
-
 Inosine mutant enzyme R84A Homo sapiens
2.4.2.1 0.14
-
 Inosine mutant enzyme S33A Homo sapiens
2.4.2.1 0.18
-
 hypoxanthine mutant enzyme M219A Homo sapiens
2.4.2.1 0.21
-
 Inosine mutant enzyme H257A Homo sapiens
2.4.2.1 0.22
-
 Inosine mutant enzyme E89A Homo sapiens
2.4.2.1 0.22
-
 phosphate reaction with inosine, mutant enzyme T242A Homo sapiens
2.4.2.1 0.28
-
 Inosine mutant enzyme H86A Homo sapiens
2.4.2.1 0.31
-
 Inosine mutant enzyme F159A Homo sapiens
2.4.2.1 0.57
-
 hypoxanthine mutant enzyme F200A Homo sapiens
2.4.2.1 1
-
 hypoxanthine mutant enzyme E201A Homo sapiens
2.4.2.1 2.1
-
 phosphate mutant enzyme Y88F Homo sapiens
2.4.2.1 2.3
-
 hypoxanthine mutant enzyme N243A Homo sapiens
2.4.2.1 2.5
-
 phosphate mutant enzymes H257A and F159A Homo sapiens
2.4.2.1 2.7
-
 phosphate reaction with inosine, mutant enzyme K244A Homo sapiens
2.4.2.1 3.5
-
 phosphate reaction with inosine, mutant enzyme N243A Homo sapiens
2.4.2.1 3.6
-
 phosphate reaction with inosine, mutant enzyme S33A Homo sapiens
2.4.2.1 4
-
 phosphate reaction with inosine, wild-type enzyme Homo sapiens
2.4.2.1 4.3
-
 phosphate mutant enzyme M219A Homo sapiens
2.4.2.1 4.5
-
 phosphate reaction with inosine, mutant enzyme F200A Homo sapiens
2.4.2.1 4.5
-
 phosphate reaction with inosine, mutant enzyme R84A Homo sapiens
2.4.2.1 6.1
-
 phosphate reaction with inosine, mutant enzyme E201A Homo sapiens
2.4.2.1 8
-
 Inosine mutant enzyme M219A Homo sapiens
2.4.2.1 8.4
-
 Inosine mutant enzyme E201A Homo sapiens
2.4.2.1 9.2
-
 phosphate reaction with inosine, mutant enzyme H86A Homo sapiens
2.4.2.1 19
-
 Inosine mutant enzyme F200A Homo sapiens
2.4.2.1 91
-
 phosphate reaction with inosine, mutant enzyme E89A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 wild-type and recombinant mutant enzymes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 blood
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 0.06
-
 Inosine mutant enzyme N243A Homo sapiens
2.4.2.1 0.09
-
 phosphate reaction with inosine, mutant enzyme N243A Homo sapiens
2.4.2.1 0.5
-
 hypoxanthine mutant enzyme R84A Homo sapiens
2.4.2.1 0.97
-
 hypoxanthine mutant enzyme H86A Homo sapiens
2.4.2.1 1.1
-
 Inosine mutant enzyme S33A Homo sapiens
2.4.2.1 1.1
-
 phosphate reaction with inosine, mutant enzyme K244A Homo sapiens
2.4.2.1 1.4
-
 hypoxanthine mutant enzyme E201A Homo sapiens
2.4.2.1 1.5
-
 phosphate reaction with inosine, mutant enzyme R84A Homo sapiens
2.4.2.1 1.8
-
 phosphate reaction with inosine, mutant enzyme E201A Homo sapiens
2.4.2.1 2.1
-
 Inosine mutant enzyme R84A Homo sapiens
2.4.2.1 2.1
-
 hypoxanthine mutant enzyme S33A Homo sapiens
2.4.2.1 4.2
-
 Inosine mutant enzyme E201A Homo sapiens
2.4.2.1 6.2
-
 phosphate reaction with inosine, mutant enzyme F200A Homo sapiens
2.4.2.1 9
-
 hypoxanthine mutant enzyme Y88F Homo sapiens
2.4.2.1 9.4
-
 Inosine mutant enzyme F200A Homo sapiens
2.4.2.1 9.4
-
 phosphate reaction with inosine, mutant enzyme H86A Homo sapiens
2.4.2.1 10
-
 hypoxanthine mutant enzyme H257A Homo sapiens
2.4.2.1 12
-
 phosphate reaction with inosine, mutant enzyme T242A Homo sapiens
2.4.2.1 13
-
 hypoxanthine mutant enzyme N243A Homo sapiens
2.4.2.1 13
-
 phosphate reaction with inosine, mutant enzyme H257A Homo sapiens
2.4.2.1 14
-
 hypoxanthine mutant enzyme F200A Homo sapiens
2.4.2.1 14
-
 Inosine mutant enzymes T242A and H86A Homo sapiens
2.4.2.1 16
-
 Inosine mutant enzyme H257A Homo sapiens
2.4.2.1 24
-
 hypoxanthine mutant enzyme F159A Homo sapiens
2.4.2.1 30
-
 phosphate reaction with inosine, mutant enzyme F159A Homo sapiens
2.4.2.1 32
-
 Inosine mutant enzyme F159A Homo sapiens
2.4.2.1 41
-
 phosphate reaction with inosine, mutant enzyme M219A Homo sapiens
2.4.2.1 46
-
 Inosine mutant enzyme E89A Homo sapiens
2.4.2.1 49
-
 Inosine mutant enzyme K244A Homo sapiens
2.4.2.1 52
-
 phosphate reaction with inosine, mutant enzyme K244A Homo sapiens
2.4.2.1 52
-
 Inosine mutant enzyme M219A Homo sapiens
2.4.2.1 53
-
 phosphate reaction with inosine, mutant enzyme E89A Homo sapiens
2.4.2.1 54
-
 Inosine mutant enzyme Y88F Homo sapiens
2.4.2.1 56
-
 phosphate wild-type enzyme Homo sapiens
2.4.2.1 56
-
 Inosine wild-type enzyme Homo sapiens
2.4.2.1 56
-
 phosphate reaction with inosine, mutant enzyme Y88F Homo sapiens
2.4.2.1 58
-
 hypoxanthine mutant enzyme T242A Homo sapiens
2.4.2.1 98
-
 hypoxanthine mutant enzyme K244A Homo sapiens
2.4.2.1 110
-
 hypoxanthine wild-type enzyme Homo sapiens
2.4.2.1 130
-
 hypoxanthine mutant enzyme M219A Homo sapiens