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Literature summary extracted from

  • Lee, J.; Filosa, S.; Bonvin, J.; Guyon, S.; Aponte, R.A.; Turnbull, J.L.
    Expression, purification, and characterization of recombinant purine nucleoside phosphorylase from Escherichia coli (2001), Protein Expr. Purif., 22, 180-188.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.1 overexpression in Escherichia coli Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.135
-
 phosphate reaction with 7-methylinosine Escherichia coli
2.4.2.1 0.167
-
 phosphate reaction with 7-methylinosine Cellulomonas sp.
2.4.2.1 0.169
-
 7-Methylinosine
-
 Cellulomonas sp.
2.4.2.1 0.247
-
 7-Methylinosine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 168000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Cellulomonas sp.
-
-
-
2.4.2.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 recombinant enzyme Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 36
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 7-methylinosine + phosphate
-
 Escherichia coli 7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 7-methylinosine + phosphate
-
 Cellulomonas sp. 7-methylhypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Escherichia coli hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Cellulomonas sp. hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 hexamer
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 55
-
 10 min, stable Cellulomonas sp.
2.4.2.1 55
-
 10 min, 20% loss of activity Escherichia coli
2.4.2.1 60
-
 10 min, about 10% loss of activity Cellulomonas sp.
2.4.2.1 60
-
 10 min, about 35% loss of activity Escherichia coli
2.4.2.1 67
-
 10 min, about 90% loss of activity Escherichia coli
2.4.2.1 67
-
 10 min, 45% loss of activity Cellulomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.1 2.01
-
 phosphate reaction with 7-methylinosine Escherichia coli
2.4.2.1 2.57
-
 7-Methylinosine
-
 Escherichia coli
2.4.2.1 27.5
-
 7-Methylinosine
-
 Cellulomonas sp.
2.4.2.1 27.8
-
 phosphate reaction with 7-methylinosine Cellulomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 8
-
-
 Cellulomonas sp.
2.4.2.1 8.8 9.3
-
 Escherichia coli