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Literature summary extracted from

  • Choi, H.S.
    Purification and partial characterization of purine nucleoside phosphorylase from Serratia marcescens (1998), Biosci. Biotechnol. Biochem., 62, 667-671.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.1 lyophilization decreases the activity by 27% Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.19
-
 guanosine
-
 Serratia marcescens
2.4.2.1 0.35
-
 deoxyguanosine
-
 Serratia marcescens
2.4.2.1 1.1
-
 phosphate
-
 Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 25817
-
 6 * 25817, electrospray ionizing mass spectrometry Serratia marcescens
2.4.2.1 27000
-
 6 * 27000, SDS-PAGE Serratia marcescens
2.4.2.1 170000
-
 gel filtration Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Serratia marcescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 additional information
-
-
 Serratia marcescens

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 4°C, pH 7.2, 10 mM Tris-HCl buffer, stable for up to 14 days Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate
-
 Serratia marcescens adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyadenosine + phosphate
-
 Serratia marcescens alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Serratia marcescens guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate
-
 Serratia marcescens hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate
-
 Serratia marcescens guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Serratia marcescens hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 hexamer 6 * 27000, SDS-PAGE Serratia marcescens
2.4.2.1 hexamer 6 * 25817, electrospray ionizing mass spectrometry Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 42
-
 stable up to Serratia marcescens
2.4.2.1 50
-
 30 min, 25% loss of activity Serratia marcescens
2.4.2.1 60
-
 30 min, 90% loss of activity, 5 mM phosphate stabilizes Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7 7.7
-
 Serratia marcescens