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Literature summary extracted from

  • Hamamoto, T.; Noguchi, T.; Midorikawa, Y.
    Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2 (1996), Biosci. Biotechnol. Biochem., 60, 1179-1180.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 1.18
-
 Inosine
-
 Geobacillus stearothermophilus
2.4.2.1 1.29
-
 guanosine
-
 Geobacillus stearothermophilus
2.4.2.2 1.38
-
 uridine
-
 Haemophilus influenzae
2.4.2.2 1.9
-
 thymidine
-
 Haemophilus influenzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 30000
-
 3 * 30000, SDS-PAGE Geobacillus stearothermophilus
2.4.2.1 90000
-
 gel filtration Geobacillus stearothermophilus
2.4.2.2 46000
-
 2 * 46000, SDS-PAGE Haemophilus influenzae
2.4.2.2 92000
-
 gel filtration Haemophilus influenzae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Geobacillus stearothermophilus
-
 TH 6-2
-
2.4.2.1 Geobacillus stearothermophilus TH 6-2
-
 TH 6-2
-
2.4.2.2 Haemophilus influenzae
-
 TH 6-2
-
2.4.2.2 Haemophilus influenzae TH 6-2
-
 TH 6-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Geobacillus stearothermophilus
2.4.2.2
-
 Haemophilus influenzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 91.6
-
-
 Geobacillus stearothermophilus
2.4.2.2 205
-
-
 Haemophilus influenzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenosine + phosphate no activity Geobacillus stearothermophilus adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate no activity Geobacillus stearothermophilus TH 6-2 adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyguanosine + phosphate 85% of the activity with inosine Geobacillus stearothermophilus guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyguanosine + phosphate 85% of the activity with inosine Geobacillus stearothermophilus TH 6-2 guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate as active as inosine Geobacillus stearothermophilus hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 deoxyinosine + phosphate as active as inosine Geobacillus stearothermophilus TH 6-2 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate 84% of the activity with guanosine Geobacillus stearothermophilus guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate 84% of the activity with guanosine Geobacillus stearothermophilus TH 6-2 guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus TH 6-2 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 xanthosine + phosphate
-
 Geobacillus stearothermophilus alpha-D-ribose 1-phosphate + xanthine
-
 ?
2.4.2.2 deoxyuridine + phosphate 147% of the activity with uridine Haemophilus influenzae uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 deoxyuridine + phosphate 147% of the activity with uridine Haemophilus influenzae TH 6-2 uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 ribofuranosyl thymine + phosphate
-
 Haemophilus influenzae thymine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 ribofuranosyl thymine + phosphate
-
 Haemophilus influenzae TH 6-2 thymine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate 99% of the activity with uridine Haemophilus influenzae thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 thymidine + phosphate 99% of the activity with uridine Haemophilus influenzae TH 6-2 thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Haemophilus influenzae uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.2 uridine + phosphate
-
 Haemophilus influenzae TH 6-2 uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 trimer 3 * 30000, SDS-PAGE Geobacillus stearothermophilus
2.4.2.2 dimer 2 * 46000, SDS-PAGE Haemophilus influenzae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 70
-
-
 Geobacillus stearothermophilus
2.4.2.2 70
-
-
 Haemophilus influenzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 70
-
 stable below Geobacillus stearothermophilus
2.4.2.2 60
-
 stable below Haemophilus influenzae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 8
-
-
 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.1 5 9 stable from pH 5.0 to pH 9.0 Geobacillus stearothermophilus
2.4.2.2 5 9 stable Haemophilus influenzae