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Literature summary extracted from

  • Milman, G.; Anton, D.L.; Weber, J.L.
    Chinese hamster purine-nucleoside phosphorylase: purification, structural, and catalytic properties (1976), Biochemistry, 15, 4967-4973.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.02
-
 hypoxanthine
-
 Cricetulus griseus
2.4.2.1 0.035
-
 guanine
-
 Cricetulus griseus
2.4.2.1 0.05
-
 Inosine
-
 Cricetulus griseus
2.4.2.1 0.2
-
 phosphate
-
 Cricetulus griseus
2.4.2.1 0.2
-
 alpha-D-ribose 1-phosphate
-
 Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 30000
-
 2 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE Cricetulus griseus
2.4.2.1 30000
-
 3 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE Cricetulus griseus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Cricetulus griseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Cricetulus griseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 kidney
-
 Cricetulus griseus
-
2.4.2.1 liver
-
 Cricetulus griseus
-
2.4.2.1 V-79 cell
-
 Cricetulus griseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 60
-
-
 Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 guanosine + phosphate
-
 Cricetulus griseus guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Cricetulus griseus hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.1 dimer 2 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE Cricetulus griseus
2.4.2.1 More enzyme behaves as a mixture of dimers of 68000 Da and trimers of 89000 Da Cricetulus griseus
2.4.2.1 trimer 3 * 30000, enzyme behaves as a mixture of dimers and trimers, SDS-PAGE Cricetulus griseus