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Literature summary extracted from

  • May, R.A.; Hoffee, P.
    Purine nucleoside phosphorylases purified from rat liver and Novikoff hepatoma cells (1979), Arch. Biochem. Biophys., 193, 398-406.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.1
-
 Inosine
-
 Rattus norvegicus
2.4.2.1 0.12
-
 guanosine
-
 Rattus norvegicus
2.4.2.1 0.52
-
 phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 29000
-
 3 * 29000, SDS-PAGE Rattus norvegicus
2.4.2.1 84000
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 liver
-
 Rattus norvegicus
-
2.4.2.1 Novikoff hepatoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 132
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 6-mercaptopurine riboside + phosphate 14% of the activity with inosine Rattus norvegicus alpha-D-ribose 1-phosphate + 6-mercaptopurine
-
 ?
2.4.2.1 guanosine + phosphate 16% of the activity with inosine Rattus norvegicus guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Rattus norvegicus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 trimer 3 * 29000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 60
-
 pH 7.4, 20 min, about 20% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.5 8
-
 Rattus norvegicus