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Literature summary extracted from

  • Machida, Y.; Nakanishi, T.
    Properties of purine nucleoside phosphorylase from Enterobacter cloacae (1981), Agric. Biol. Chem., 45, 1801-1807.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0578
-
 phosphate
-
 Enterobacter cloacae
2.4.2.1 0.09
-
 Inosine
-
 Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 87000
-
 gel filtration Enterobacter cloacae

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Alcaligenes faecalis
-
 KY3106
-
2.4.2.1 Alcaligenes faecalis KY3106
-
 KY3106
-
2.4.2.1 Arthrobacter nucleogenes
-
 KY3168
-
2.4.2.1 Arthrobacter nucleogenes KY3168
-
 KY3168
-
2.4.2.1 Bacterium cadaveris
-
 KY3402
-
2.4.2.1 Bacterium cadaveris KY3402
-
 KY3402
-
2.4.2.1 Cellulomonas cellasea
-
 KY3491
-
2.4.2.1 Cellulomonas cellasea KY3491
-
 KY3491
-
2.4.2.1 Corynebacterium vitaeruminis
-
 KY3459
-
2.4.2.1 Corynebacterium vitaeruminis KY3459
-
 KY3459
-
2.4.2.1 Enterobacter cloacae
-
 KY3074
-
2.4.2.1 Enterobacter cloacae KY3074
-
 KY3074
-
2.4.2.1 Micrococcus luteus
-
 KY 3760
-
2.4.2.1 Micrococcus luteus KY 3760
-
 KY 3760
-
2.4.2.1 Proteus mirabilis
-
 KY4057
-
2.4.2.1 Proteus mirabilis KY4057
-
 KY4057
-
2.4.2.1 Xanthomonas campestris
-
 KY4208
-
2.4.2.1 Xanthomonas campestris KY4208
-
 KY4208
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1 partial Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 additional information
-
-
 Enterobacter cloacae
2.4.2.1 7.9
-
-
 Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 deoxyadenosine + phosphate 112.9% of the activity with inosine Enterobacter cloacae alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyadenosine + phosphate 112.9% of the activity with inosine Enterobacter cloacae KY3074 alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 guanosine + phosphate 47.4% of the reaction with inosine Enterobacter cloacae guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate 47.4% of the reaction with inosine Enterobacter cloacae KY3074 guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate the equilibrium is reached when approximately 14% of inosine is phosphorylated Enterobacter cloacae hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate the equilibrium is reached when approximately 14% of inosine is phosphorylated Enterobacter cloacae KY3074 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 xanthosine + phosphate 43.5% of the activity with inosine Enterobacter cloacae alpha-D-ribose 1-phosphate + xanthine
-
 ?
2.4.2.1 xanthosine + phosphate 43.5% of the activity with inosine Enterobacter cloacae KY3074 alpha-D-ribose 1-phosphate + xanthine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 50
-
-
 Enterobacter cloacae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.1 30 70 30°C: about 75% of maximal activity, 70°C: about 80% of maximal activity Enterobacter cloacae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 60
-
 30 min, stable Enterobacter cloacae
2.4.2.1 70
-
 30 min, about 90% loss of activity Enterobacter cloacae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.5 8.5
-
 Enterobacter cloacae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 6 10 pH 6.0: about 60% of maximal activity, pH 10.0: about 75% of maximal activity Enterobacter cloacae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.1 6.5
-
 80°C, 30 min, about 75% loss of activity Enterobacter cloacae
2.4.2.1 7 7.3 80°C, 30 min, stable Enterobacter cloacae
2.4.2.1 8
-
 80°C, 30 min, about 35% loss of activity Enterobacter cloacae