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Literature summary extracted from

  • Utagawa, T.; Morisawa, H.; Yamanaka, S.; Yamazaki, A.; Yoshinaga, F.; Hirose, Y.
    Properties of nucleoside phosphorylase from Enterobacter aerogenes (1985), Agric. Biol. Chem., 49, 3239-3246.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.8
-
 Inosine 40°C Klebsiella aerogenes
2.4.2.1 2.2
-
 Inosine 60°C Klebsiella aerogenes
2.4.2.3 0.7
-
 uridine at 40°C Klebsiella aerogenes
2.4.2.3 1.8
-
 uridine at 60°C Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Klebsiella aerogenes
-
-
-
2.4.2.3 Klebsiella aerogenes
-
 AJ 11125
-
2.4.2.3 Klebsiella aerogenes AJ 11125
-
 AJ 11125
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 partial Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 additional information
-
-
 Klebsiella aerogenes
2.4.2.3 97.83
-
-
 Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.4.2.1 30°C, 1 month, less than 20% loss of activity Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 adenine arabinoside + phosphate 49% of the activity with adenosine Klebsiella aerogenes alpha-D-arabinose 1-phosphate + adenine
-
 ?
2.4.2.1 adenosine + phosphate best substrate Klebsiella aerogenes adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 adenosine + phosphate best substrate Klebsiella aerogenes AJ 11125 adenine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 deoxyadenosine + phosphate 2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine Klebsiella aerogenes alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyadenosine + phosphate 2'-deoxyadenosine, 65% of the activity with adenosine. 3'-deoxyadenosine, 8% of the activity with adenosine Klebsiella aerogenes AJ 11125 alpha-D-ribose 1-phosphate + adenine
-
 ?
2.4.2.1 deoxyinosine + phosphate 2'-deoxyinosine, 43% of the activity with adenosine Klebsiella aerogenes hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 deoxyinosine + phosphate 2'-deoxyinosine, 43% of the activity with adenosine Klebsiella aerogenes AJ 11125 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate 34% of the activity with adenosine Klebsiella aerogenes hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate equilibrium when 65% of inosine has been phosphorylated Klebsiella aerogenes hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate 34% of the activity with adenosine Klebsiella aerogenes AJ 11125 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate equilibrium when 65% of inosine has been phosphorylated Klebsiella aerogenes AJ 11125 hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.3 2'-deoxyuridine + phosphate 18% of the activity with uridine Klebsiella aerogenes uracil + deoxyribose 1-phosphate
-
 ?
2.4.2.3 additional information no substrate: cytidine Klebsiella aerogenes ?
-
 ?
2.4.2.3 thymidine + phosphate 22% of the activity with uridine Klebsiella aerogenes thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uracil arabinoside + phosphate 10% of the activity with uridine Klebsiella aerogenes uracil + arabinose-1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Klebsiella aerogenes uracil + alpha-D-ribose 1-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 60
-
-
 Klebsiella aerogenes
2.4.2.3 65
-
-
 Klebsiella aerogenes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.3 40 80 40°C: about 40% of activity maximum, 80°C: about 10% of activity maximum Klebsiella aerogenes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 30
-
 1 month, less than 20% loss of activity Klebsiella aerogenes
2.4.2.1 60
-
 half-life: around 1 week Klebsiella aerogenes
2.4.2.3 30
-
 1 month, 20% loss of activity Klebsiella aerogenes
2.4.2.3 60
-
 half-life: around 1 week Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 6.8
-
-
 Klebsiella aerogenes
2.4.2.3 8.5
-
-
 Klebsiella aerogenes

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.3 6.5 10.5 pH 6.5: about 50% of activity maximum, pH 10.5: about 65% of activity maximum Klebsiella aerogenes