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Literature summary extracted from

  • Lewis, A.S.; Lowy, B.A.
    Human erythrocyte purine nucleoside phosphorylase: molecular weight and physical properties. A Theorell-Chance catalytic mechanism (1979), J. Biol. Chem., 254, 9927-9932.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 alpha-D-ribose 1-phosphate
-
 Homo sapiens
2.4.2.1 guanine
-
 Homo sapiens
2.4.2.1 guanosine product inhibition Homo sapiens
2.4.2.1 phosphate product inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.0308
-
 guanine
-
 Homo sapiens
2.4.2.1 0.039
-
 hypoxanthine
-
 Homo sapiens
2.4.2.1 0.0443
-
 guanosine
-
 Homo sapiens
2.4.2.1 0.0443
-
 deoxyguanosine
-
 Homo sapiens
2.4.2.1 0.05
-
 Inosine
-
 Homo sapiens
2.4.2.1 0.167
-
 6-mercaptoguanosine
-
 Homo sapiens
2.4.2.1 0.228
-
 alpha-D-ribose 1-phosphate reaction with guanine Homo sapiens
2.4.2.1 13.1
-
 phosphate reaction with guanosine Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 32000
-
 2 * 32000, SDS-PAGE Homo sapiens
2.4.2.1 67000
-
 equilibrium sedimentation, gel filtration, amino acid analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.1 purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate Theorell-Chance mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.1 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 55
-
 reaction with guanosine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 6-mercaptoguanosine + phosphate
-
 Homo sapiens alpha-D-ribose 1-phosphate + 6-mercaptoguanine
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Homo sapiens guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Homo sapiens guanine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.1 monomer 2 * 32000, SDS-PAGE Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.1 0.01
-
 guanosine with guanine as variable substrate and ribose 1-phosphate as fixed substrate Homo sapiens
2.4.2.1 0.0125
-
 guanine with guanosine as variable substrate and phosphate as fixed substrate Homo sapiens