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Literature summary extracted from

  • Hellwege, E.M.; Gritscher, D.; Willmitzer, L.; Heyer, A.G.
    Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom disks (1997), Plant J., 12, 1057-1065.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.99 subcloned into a pUC18 derivative and expressed in tobacco protoplasts under the control of the cauliflower mosaic virus 35S RNA promotor Cynara cardunculus var. scolymus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.99 Ag+
-
 Cynara cardunculus var. scolymus
2.4.1.99 Cu2+
-
 Cynara cardunculus var. scolymus
2.4.1.99 Zn2+
-
 Cynara cardunculus var. scolymus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.99 sucrose + sucrose Cynara cardunculus var. scolymus the enzyme is the entry point of fructan synthesis, giving rise to the accumulation of 1-kestose and nystose on expression in plant storage organs D-glucose + 1F-beta-D-fructosylsucrose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Cynara cardunculus var. scolymus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.99 disc
-
 Cynara cardunculus var. scolymus
-
2.4.1.99 primary root
-
 Cynara cardunculus var. scolymus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 sucrose + sucrose
-
 Cynara cardunculus var. scolymus D-glucose + 1F-beta-D-fructosylsucrose 1-kestose is the only fructan product ?
2.4.1.99 sucrose + sucrose the enzyme is the entry point of fructan synthesis, giving rise to the accumulation of 1-kestose and nystose on expression in plant storage organs Cynara cardunculus var. scolymus D-glucose + 1F-beta-D-fructosylsucrose
-
 ?