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Literature summary extracted from

  • Thomson, K.S.
    Purification of UDOgalactose:sn-glycerol-3-phosphate alpha-D-galactosyltransferase from Poterioochromonas malhamensis (1983), Biochim. Biophys. Acta, 759, 154-159.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.4.1.96 cetyltrimethylammonium bromide, optimal concentration for stabilization, 0.075% w/v at pH 8.5, 0.1% w/v at pH 7.2, 0.2 w/v at pH 6.2 Poterioochromonas malhamensis
2.4.1.96 glycerol, 30% v/v, stabilizes the enzyme at pH 7.8 but not at lower or higher pH values Poterioochromonas malhamensis
2.4.1.96 unstable to repeated freezing and thawing Poterioochromonas malhamensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.96 0.01
-
 UDPgalactose
-
 Poterioochromonas malhamensis
2.4.1.96 0.02
-
 sn-glycerol 3-phosphate
-
 Poterioochromonas malhamensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.96 68000
-
 gel filtration Poterioochromonas malhamensis
2.4.1.96 70000
-
 1 * 70000, SDS-PAGE Poterioochromonas malhamensis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.96 Poterioochromonas malhamensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.96
-
 Poterioochromonas malhamensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.96 198
-
-
 Poterioochromonas malhamensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.96 UDPgalactose + sn-glycerol 3-phosphate
-
 Poterioochromonas malhamensis UDP + alpha-D-galactosyl sn-glycerol 3-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.96 monomer 1 * 70000, SDS-PAGE Poterioochromonas malhamensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.96 7 8
-
 Poterioochromonas malhamensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.96 6 9 about 50% of activity maximum at pH 6 and pH 9 Poterioochromonas malhamensis