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Literature summary extracted from

  • Haltiwanger, R.S.; Blomberg, M.A.; Hart, G.W.
    Glycosylation of nuclear and cytoplasmic proteins. Purification and characterization of a uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase (1992), J. Biol. Chem., 267, 9005-9013.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.255 CDP
-
 Rattus norvegicus
2.4.1.255 KCl 50 mM causes 66% inhibition Rattus norvegicus
2.4.1.255 NaCl 50 mM causes 66% inhibition Rattus norvegicus
2.4.1.255 thiouridine diphosphate SUDP Rattus norvegicus
2.4.1.255 UDP UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity Rattus norvegicus
2.4.1.255 UDP-GalNAc UMP and UDP-GalNAc are 100fold less potent than UDP, UTP, and UDP-GlcNAc Rattus norvegicus
2.4.1.255 UDP-GlcNAc UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity Rattus norvegicus
2.4.1.255 UMP UMP and UDP-GalNAc are 100fold less potent than UDP, UTP, and UDP-GlcNAc Rattus norvegicus
2.4.1.255 UTP UDP, UTP, and UDP-GlcNAc are all equally potent inhibitors of the activity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.255 0.000545
-
 UDP-GlcNAc extremely high affinity Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.255 cytosol
-
 Rattus norvegicus 5829
-
2.4.1.255 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.255 Mg2+ manganese choride 5 mM, essential for binding, although divalent cations are not required for activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.255 78000
-
 2 * 110000, 1 * 78000 (alpha2beta) Rattus norvegicus
2.4.1.255 110000
-
 2 * 110000, 1 * 78000 (alpha2beta) Rattus norvegicus
2.4.1.255 340000
-
 on molecular sieve chromatography Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.255 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.255 affinity chromatography, at pH 6.0-6.5 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.255 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.255 0.00111
-
 last purification step Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.255 Purified enzyme is stable and can be stored for several months at -20°C with negligible loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.255 UDP-GlcNAc + YSDSPSTST highly specific synthetic peptide assay, affinity of the enzyme for UDP-GlcNAc is high and for the peptide substrate, YSDSPSTST, is only moderate Rattus norvegicus UDP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.255 heterotrimer 2 * 110000, 1 * 78000 (alpha2beta) Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.255 O-GlcNAc transferase
-
 Rattus norvegicus
2.4.1.255 uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.255 6
-
 about Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.255 6 7.5 activity remaining fairly high up to pH 7.5 while dropping rapidly below 6 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.255 0.00018
-
-
 Rattus norvegicus UDP-GlcNAc
2.4.1.255 0.0002
-
-
 Rattus norvegicus UDP
2.4.1.255 0.0002
-
-
 Rattus norvegicus UTP
2.4.1.255 0.016
-
-
 Rattus norvegicus UDP-GalNAc
2.4.1.255 0.018
-
-
 Rattus norvegicus UDP
2.4.1.255 0.019
-
-
 Rattus norvegicus UMP